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Morphological control of merlin-Rac antagonism in proliferation-promoting signaling.

Weiss BG, Keth JM, Bhatt K, Doyal M, Hahn KM, Noh J, Isogai T, Danuser G
Sci Signal. 2025 May 20;18(887):eadk0922. doi: 10.1126/scisignal.adk0922. Epub 2025 May 20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
241366pLVXCMV100-mNeonGreen-PBDLentiviral expression of mNeonGreen-tagged PAK1 p21-binding domain (PBD)
241367pLVX-IRES-NEO-NF2-18-SNAPLentiviral expression of SNAP-tagged NF2
241368pX458-ARHGEF7/B-PixMammalian expression of SpCas9 and gRNA targeting ARHGEF7 (β-Pix)
241369pLVX-IRES-PURO-RAC1-R66ALentiviral expression of mutant Rac1
241370pLVX-IRES-PURO-RAC1-Y64FLentiviral expression of mutant Rac1
241371pBabe-Tre-Rac1-P29S (FLARE)Dox-inducible expression of Rac1-P29S FLARE sensor
241401pLVX-IRES-NEO-NF2(1-27)-SNAP-tagLentiviral expression of SNAP-tagged NF2 fragment (aa 1-27)

Antibodies from Article