Measuring and manipulating localized translation of erm-1 in the C. elegans embryo.
van der Salm E, Koelewijn E, Schroeder M, van der Maas E, Jarosinska O, Eeken M, Ruijtenberg S
Development. 2025 May 15;152(10):dev204435. doi: 10.1242/dev.204435. Epub 2025 May 19.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 245120 | pSRG12 (eft-3p::24xGCN4::t2a::tagbfp::h2b::tbb-2 3’UTR) | SunTag reporter for live imaging of translation (when combined with scFv::GFP expression) in C. elegans |
| 245121 | pSRG11 (eft-3p::scfv(glo)::gfp(smu-1 introns)::tbb-2 3’UTR) | Optimized SunTag antibody for expression in C. elegans. Useful for visualization of translation of GCN4 encoding mRNAs or for visualization of GCN4-tagged proteins |