CRISPR/Cas9-compatible plasmids enabling seven dominant genetic selection methods for the human fungal pathogen Cryptococcus neoformans.
Boucher MJ, Madhani HD
Microbiol Spectr. 2025 Sep 25:e0193525. doi: 10.1128/spectrum.01935-25.
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PubMed
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 241719 | pBHM2672 | Integration of DNA into Cryptococcus neoformans Safe Haven 1 locus, contains BLE marker for selection on phleomycin |
| 241720 | pBHM2673 | Integration of DNA into Cryptococcus neoformans Safe Haven 1 locus, contains BSD marker for selection on blasticidin S |
| 241722 | pBHM2675 | Integration of DNA into Cryptococcus neoformans Safe Haven 1 locus, contains ptxD marker for selection on media containing phosphite as a sole phosphorus source |
| 241723 | pBHM2678 | Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains BLE marker for selection on phleomycin |
| 241724 | pBHM2679 | Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains BSD marker for selection on blasticidin S |
| 241725 | pBHM2680 | Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains BSR marker for selection on blasticidin S |
| 241726 | pBHM2681 | Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains ptxD marker for selection on media containing phosphite as a sole phosphorus source |
| 241727 | pBHM2674 | Integration of DNA into Cryptococcus neoformans Safe Haven 1 locus, contains BSR marker for selection on blasticidin S |