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Establishing synthetic ribbon-type active zones in a heterologous expression system

Kapoor R, Schwenzer N, Dresbach T, Lehnart SE, Moser T
eLife13:RP98254 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
241988pAAV-CMV-HBA-RIBEYE-eGFPHuman RIBEYE cDNA cloned inframe into a pAAV-GFP vector driven by hybrid CMV-enhancer-human-beta-actin promoter (CMV-HBA) and containing downstream WPRE, Ampicillin Resistance
241989f(syn)w-CMV-mKate2-p2A-RimBP2Insert coding for mouse RBP2 (accession ID NP_001074857.1) with an N-terminal mKATE2 with a p2A cleavage site cloned inframe into a f(syn)w-mKATE2-p2A.
241990pAAV-CMV-RBP2Mouse RBP2 (accession ID NP_001074857.1), CMV promoter-enhancer, downstream WPRE.

Antibodies from Article