Tracking transcription-translation coupling in real time.
Qureshi NS, Duss O
Nature. 2025 Jan;637(8045):487-495. doi: 10.1038/s41586-024-08308-w. Epub 2024 Dec 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 242075 | pAC005 | Expresses NusA as fusion with 6xHis-SUMO |
| 242076 | pNQ054 | Expresses NusG as fusion with 6xHis-SUMO |
| 242077 | pNQ055 | Expresses MetRS (truncated at K548) as fusion with 6xHis-SUMO |
| 242078 | pNQ056 | Expresses MTF as fusion with 6xHis-SUMO |
| 242079 | pNQ058 | Expresses rS1 as fusion with 6xHis-SUMO |
| 242080 | pNQ116 | Expresses NusG (M1-P124) as fusion with 6xHis-SUMO |
| 242081 | pNQ117 | Expresses NusG-F165A as fusion with 6xHis-SUMO |
| 242082 | pNQ071 | Expresses E. coli RNAP (gene inserts: rpoA-rpoB-Nter-ybbR-rpoC-6xHis and rpoZ) |
| 242083 | pNQ078 | Expresses E. coli RNAP (gene inserts: rpoA-rpoB-rpoC-Cter-ybbR--6xHis and rpoZ) |