Replication-dependent histone labeling dissects the physical properties of euchromatin/heterochromatin in living human cells.
Minami K, Nakazato K, Ide S, Kaizu K, Higashi K, Tamura S, Toyoda A, Takahashi K, Kurokawa K, Maeshima K
Sci Adv. 2025 Mar 28;11(13):eadu8400. doi: 10.1126/sciadv.adu8400. Epub 2025 Mar 28.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 244239 | pX330_H3.2 | Expresses SpCas9 and a sgRNA targeting the human histone H3.2 loci for knock-in. |
| 244240 | pGEM_H3.2-HaloTag | CRISPR/Cas9 donor plasmid to tag human histone H3.2 with Halo tag |
| 244241 | pX330_mouseH3.1 | Expresses SpCas9 and a sgRNA targeting the mouse histone H3.1 loci for knock-in. |
| 244242 | pGEM-T_mouseH3.1-HaloTag | CRISPR/Cas9 donor plasmid to tag mouse histone H3.1 with Halo tag |
| 244243 | pX330_CDC45 | Expresses SpCas9 and a sgRNA targeting the human CDC45 loci for knock-in. |