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Engineering of extracellular vesicles for efficient intracellular delivery of multimodal therapeutics including genome editors.

Liang X, Gupta D, Xie J, Van Wonterghem E, Van Hoecke L, Hean J, Niu Z, Ghaeidamini M, Wiklander OPB, Zheng W, Wiklander RJ, He R, Mamand DR, Bost J, Zhou G, Zhou H, Roudi S, Estupinan HY, Radler J, Zickler AM, Gorgens A, Hou VWQ, Slovak R, Hagey DW, de Jong OG, Uy AG, Zong Y, Mager I, Perez CM, Roberts TC, Carter D, Vader P, Esbjorner EK, de Fougerolles A, Wood MJA, Vandenbroucke RE, Nordin JZ, El Andaloussi S
Nat Commun. 2025 Apr 29;16(1):4028. doi: 10.1038/s41467-025-59377-y. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
247722pLEX-CD63-Intein-CreExpresses EV-Sorting domain CD63 and Cre recombinase, separated by self-cleaving intein
247729pLEX-VSV-G-Foldon-Intein-CreExpresses VSV-G, Foldon and Cre recombinase, separated by self-cleaving intein
247730pLEX-CD63-Intein-Cas9Expresses EV-Sorting domain CD63 and Cas9, separated by self-cleaving intein
247731pLEX-VSV-G-Foldon-Intein-Cas9Expresses VSV-G, Foldon and Cas9, separated by self-cleaving intein

Antibodies from Article