A direct experimental test of Ohno's hypothesis.

Mihajlovic L, Iyengar BR, Baier F, Barbier I, Iwaszkiewicz J, Zoete V, Wagner A, Schaerli Y
eLife. 2025 Apr 2;13:RP97216. doi: 10.7554/eLife.97216. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
223514	pAND-MCS	Plasmid backbone with multiple cloning site for two genes in opposing directions (convergently transcribed)
223515	pDUP	cogfp under control of Ptac
223516	pDUP1	inactive cogfp under control of Ptet, cogfp under control of Ptac
223517	pDUP2	cogfp copy 1 under control of Ptet, cogfp copy 2 under control of Ptac