Mitochondrial complexity is regulated at ER-mitochondria contact sites via PDZD8-FKBP8 tethering.
Nakamura K, Aoyama-Ishiwatari S, Nagao T, Paaran M, Obara CJ, Sakurai-Saito Y, Johnston J, Du Y, Suga S, Tsuboi M, Nakakido M, Tsumoto K, Kishi Y, Gotoh Y, Kwak C, Rhee HW, Seo JK, Kosako H, Potter C, Carragher B, Lippincott-Schwartz J, Polleux F, Hirabayashi Y
Nat Commun. 2025 Apr 17;16(1):3401. doi: 10.1038/s41467-025-58538-3.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 253950 | pCAG-mPDZD8-3xFLAG (1-1147) | Mammalian Expression of 3xFLAG-tagged mouse PDZD8 |
| 253951 | pCAG-mPDZD8_TMSMPC2nPDZ-3xFLAG (1-450) | Mammalian expression of 3xFLAG-tagged mouse PDZD8 N-terminal region (TM-SMP-C2n-PDZ; aa 1-450) |
| 253953 | pCAG-TMSMPdC2n-3xFLAG (1-300) | Mammalian expression of 3xFLAG-tagged mouse PDZD8 N-terminal truncation (TM-SMP-deletedC2n; aa 1-300) |
| 253955 | pCAG-mPDZD8_SMPC2nPDZ-3xFLAG (1, 30-450) | Mammalian expression of 3xFLAG-tagged mouse PDZD8 N-terminal SMP-C2n-PDZ construct (aa 30-450) |
| 253956 | pCAG-mPDZD8_PDZC2c-3xFLAG (1, 354-840) | Mammalian expression of 3xFLAG-tagged mouse PDZD8 central region (PDZ-C2c; aa 354-840) |
| 253957 | pCAG-mPDZD8_C1CC-3xFLAG (1, 841-1147) | Mammalian expression of 3xFLAG-tagged mouse PDZD8 C-terminal region (C1-CC; aa 841-1147) |
| 253958 | pCAG-mFKBP8 | Mammalian Expression of mouse FKBP8 |
| 253959 | pCAG-mFKBP8(N403K) | Mammalian Expression of mouse FKBP8N403K |
| 253960 | pCAG-HA-mFKBP8 | Mammalian Expression of HA-tagged mouse FKBP8 |
| 253962 | pGEX4T1-GST-Thrombin cleavage site-hPDZD8(1,28-506)-HA | Bacterial expression of GST-tagged human PDZD8 (aa 28-506) with thrombin cleavage site and C-terminal HA tag for recombinant protein purification. |
| 253963 | pLKO-shFKBP8#2380 | Lentiviral shRNA-mediated knockdown of mouse FKBP8 |
| 253964 | pCAG-Venus-mFKBP8(N403K) | Mammalian Expression of Venus-fused mouse FKBP8N403K |
| 253966 | FUW-CreERT2-P2A-NeoR | Lentiviral expression of tamoxifen-inducible CreERT2 with neomycin (G418) resistance (P2A-NeoR) |
| 253968 | px459ver2.0-FKBP8-gRNA1 (for mouse) | CRISPR/Cas9 (PX459) plasmid expressing gRNA N-terminal knock-in of mouse Fkbp8. |
| 253969 | pCAG-BiP-mtagBFP2-KDEL | Mammalian expression of ER marker |
| 253970 | pCAG-YFP-ActA | Mammalian expression of mitochondrial marker |
| 253973 | pCAG-hPDZD8_cterm-HaloTag-P2A-Neo | CRISPR donor vector for C-terminal tagging of human PDZD8 with HaloTag and neomycin resistance (P2A-Neo). |
| 253974 | pCAG-hPDZD8(1,28-)-Flag | Mammalian expression of FLAG-tagged human PDZD8 Cytosolic region |
| 253975 | YT210-FKBP8-gRNA3 | Mammalian expression of gRNA targeting mouse Fkbp8 (for CRISPR knockout). |
| 253977 | YT210-FKBP8-gRNA5 | Mammalian expression of gRNA targeting mouse Fkbp8 (for CRISPR knockout). |