Precision genome editing and in-cell measurements of oxidative DNA damage repair enable functional and mechanistic characterization of cancer-associated MUTYH variants.

Vasquez CA, Osgood NRB, Zepeda MU, Sandel DK, Cowan QT, Peiris MN, Donoghue DJ, Komor AC
Nucleic Acids Res. 2025 Mar 20;53(6):gkaf037. doi: 10.1093/nar/gkaf037. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
219807	pCAV035_mCherry_P2A_fsEGFP_GGsite	Fluorescent Reporter for MUTYH-Specific Activity; Containing BsaI Recognition Sequence at Frameshifted EGFP; For OG:A Oligo Insertion at Golden Gate (GG) Site at Codon 34