Probing condensate microenvironments with a micropeptide killswitch.
Zhang Y, Stoppelkamp I, Fernandez-Pernas P, Allram M, Charman M, Magalhaes AP, Piedavent-Salomon M, Sommer G, Sung YC, Meyer K, Grams N, Halko E, Dongre S, Meierhofer D, Malszycki M, Ilik IA, Aktas T, Kraushar ML, Vastenhouw N, Weitzman MD, Grebien F, Niskanen H, Hnisz D
Nature. 2025 Jun 4. doi: 10.1038/s41586-025-09141-5.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 237619 | pRK5_msfGFP-HMGB1-Mut-FtoED | For overexpression of msfGFP-HMGB1-Mut-FtoED |
| 237620 | pRK5_msfGFP-HMGB1-Mut-FtoA | For overexpression of msfGFP-HMGB1-Mut-FtoA |
| 237621 | pRK5_msfGFP-HMGB1-Mut-FtoG | For overexpression of msfGFP-HMGB1-Mut-FtoG |
| 237622 | pRK5_msfGFP-HMGB1-Mut-0F | For overexpression of msfGFP-HMGB1-Mut-0F |
| 237623 | pRK5_msfGFP-HMGB1-Mut-F12A | For overexpression of msfGFP-HMGB1-Mut-F12A |
| 237624 | pRK5_msfGFP-HMGB1-Mut-F13A | For overexpression of msfGFP-HMGB1-Mut-F13A |
| 237625 | pRK5_msfGFP-HMGB1-Mut-deltaKS 3F | For overexpression of msfGFP-HMGB1-Mut-deltaKS 3F |
| 237626 | pRK5_msfGFP-HMGB1-Mut-11G3F3G | For overexpression of msfGFP-HMGB1-Mut-11G3F3G |
| 237627 | pRK5_msfGFP-HMGB1-Mut-C16toA | For overexpression of msfGFP-HMGB1-Mut-C16toA |
| 237628 | pRK5_msfGFP-HMGB1-Mut-MtoED | For overexpression of msfGFP-HMGB1-Mut-MtoED |
| 237629 | pET22b_msfGFP_stag | For expression of msfGFP_stag in Ecoli |
| 237630 | pET22b_msfGFP_stag_KS | For expression of msfGFP_stag_KS in Ecoli |
| 237631 | pET22b_msfGFP_stag_KS(FtoG) | For expression of msfGFP_stag_KS(FtoG) in Ecoli |
| 237632 | pUC19_msfGFP-TCOF1-repair-template | For knock-in msfGFP to TCOF1 locus |
| 237633 | pX458_mCherry-TCOF1-gRNA | Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to TCOF1 locus |
| 237634 | pX458_mCherry-HP1a-gRNA-1 | Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to HP1a locus |
| 237635 | pX458_mCherry-HP1a-gRNA-2 | Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to HP1a locus |
| 237636 | pRK5_mEGFP-EWS-FLI1 | For overexpression of mEGFP-EWS-FLI1 |
| 237637 | pRK5_mEGFP-SS18-SSX2 | For overexpression of mEGFP-SS18-SSX2 |
| 237638 | pRK5_mEGFP-CRTC1-MAML2 | For overexpression of mEGFP-CRTC1-MAML2 |
| 237639 | pRK5_mEGFP-NUP98-HOXA9 | For overexpression of mEGFP-NUP98-HOXA9 |
| 237640 | pRK5_mEGFP-NUP98-DDX10 | For overexpression of mEGFP-NUP98-DDX10 |
| 237641 | pRK5_mEGFP-NONO-TFE3 | For overexpression of mEGFP-NONO-TFE3 |
| 237642 | pRK5_mEGFP-TAZ-CAMTA1 | For overexpression of mEGFP-TAZ-CAMTA1 |
| 237643 | pRK5_mEGFP-PAX-FOXO1 | For overexpression of mEGFP-PAX-FOXO1 |
| 237644 | pRK5_mEGFP-NUP98-DDX10-KS | For overexpression of mEGFP-NUP98-DDX10-KS |
| 237645 | pRK5_mEGFP-NUP98-DDX10-KS-FtoG | For overexpression of mEGFP-NUP98-DDX10-KS-FtoG |
| 237646 | pRK5_mCherry-CRM1 | For overexpression of mCherry-CRM1 |
| 237647 | pRK5_mEGFP-NUP98-DDX10-Mut1 | For overexpression of mEGFP-NUP98-DDX10-Mut1 |
| 237648 | pRK5_mEGFP-NUP98-DDX10-Mut2 | For overexpression of mEGFP-NUP98-DDX10-Mut2 |
| 237649 | pRK5_msfGFP-HMGB1-Shuffled 1 | For overexpression of msfGFP-HMGB1-Shuffled 1 |
| 237650 | pRK5_msfGFP-HMGB1-Shuffled 2 | For overexpression of msfGFP-HMGB1-Shuffled 2 |
| 237651 | pRK5_msfGFP-HMGB1-Shuffled 3 | For overexpression of msfGFP-HMGB1-Shuffled 3 |
| 237652 | pRK5_msfGFP-HMGB1-Shuffled 4 | For overexpression of msfGFP-HMGB1-Shuffled 4 |
| 237653 | pRK5_msfGFP-HMGB1-Shuffled 5 | For overexpression of msfGFP-HMGB1-Shuffled 5 |
| 237654 | pRK5_msfGFP-HMGB1-Shuffled 6 | For overexpression of msfGFP-HMGB1-Shuffled 6 |
| 237655 | pRK5_msfGFP-HMGB1-Shuffled 7 | For overexpression of msfGFP-HMGB1-Shuffled 7 |
| 237656 | pRK5_msfGFP-HMGB1-Shuffled 8 | For overexpression of msfGFP-HMGB1-Shuffled 8 |
| 237657 | pRK5_msfGFP-HMGB1-Shuffled 9 | For overexpression of msfGFP-HMGB1-Shuffled 9 |
| 237658 | pRK5_msfGFP-HMGB1-Shuffled 10 | For overexpression of msfGFP-HMGB1-Shuffled 10 |
| 237659 | pRK5_msfGFP-HMGB1-KS-FtoY | For overexpression of msfGFP-HMGB1-KS-FtoY |
| 237660 | pRK5_msfGFP-HMGB1-KS-FtoW | For overexpression of msfGFP-HMGB1-KS-FtoW |
| 237661 | PB_TetON_EGFP-NPM1 | For integration of EGFP-NPM1 |
| 237662 | PB_TetON_EGFP-NPM1-KS | For integration of EGFP-NPM1-KS |
| 237663 | PB_TetON_EGFP-NPM1-KS-FtoG | For integration of EGFP-NPM1-KS-FtoG |
| 237664 | PB_TetON_EGFP-NPM1-KS-FtoA | For integration of EGFP-NPM1-KS-FtoA |
| 237665 | PB_TetON_EGFP-NPM1-KS-0F | For integration of EGFP-NPM1-KS-0F |
| 237666 | pRK5_msfGFP-HMGB1-TDP43HP | For overexpression of msfGFP-HMGB1-TDP43HP |
| 237667 | pRK5_mEGFP-NPM1 | For overexpression of mEGFP-NPM1 |
| 237668 | pRK5_mEGFP-NPM1-KS | For overexpression of mEGFP-NPM1-KS |
| 237669 | pRK5_mEGFP-NPM1-TDP43HP | For overexpression of mEGFP-NPM1-TDP43HP |
| 237670 | pET22b-msfGFP-NPM1 | For expression of msfGFP-NPM1 in Ecoli |
| 237671 | pRK5_mEGFP-EWS-FLI1-KS | For overexpression of mEGFP-EWS-FLI1-KS |
| 237672 | pRK5_mEGFP-CRTC1-MAML2-KS | For overexpression of mEGFP-CRTC1-MAML2-KS |
| 237673 | pRK5_mEGFP-NUP98-HOXA9-KS | For overexpression of mEGFP-NUP98-HOXA9-KS |
| 237674 | pRK5_mEGFP-YAP-MAML2 | For overexpression of mEGFP-YAP-MAML2 |
| 237675 | pRK5_mEGFP-YAP-MAMLD1 | For overexpression of mEGFP-YAP-MAMLD1 |
| 237676 | pRK5_mEGFP-YAP-MAMLD1-KS | For overexpression of mEGFP-YAP-MAMLD1-KS |
| 237677 | pRK5_mEGFP-TAZ-CAMTA1-KS | For overexpression of mEGFP-TAZ-CAMTA1-KS |
| 237678 | pRK5_mEGFP-SS18-SSX1 | For overexpression of mEGFP-SS18-SSX1 |
| 237679 | pRK5_mEGFP-SS18-SSX1-KS | For overexpression of mEGFP-SS18-SSX1-KS |
| 237680 | pRK5_mEGFP-SS18-SSX2-KS | For overexpression of mEGFP-SS18-SSX2-KS |
| 237681 | pRK5_mEGFP-NONO-TFE3-KS | For overexpression of mEGFP-NONO-TFE3-KS |
| 237682 | pUC19_msfGFP-EWSR1-repair-tempate | For knock-in msfGFP to EWSR1 locus |
| 237683 | pX458_mCherry-EWSR1-gRNA | Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to EWSR1 locus |
| 237684 | pUC19_msfGFP-FUS-repair-tempate | For knock-in msfGFP to FUS locus |
| 237685 | pX458_mCherry-FUS-gRNA | Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to FUS locus |
| 237686 | p52K-GFP-linker-KS | For overexpression of 52K-GFP-linker-KS |
| 237687 | p52K-GFP-linker-KS-FtoA | For overexpression of 52K-GFP-linker-KS-FtoA |
| 237688 | p52K-KS | For overexpression of 52K-KS |
| 237689 | p52K-KS-FtoA | For overexpression of 52K-KS-FtoA |
| 237690 | p52K-GFP-KS | For overexpression of 52K-GFP-KS |
| 237691 | p52K-GFP-KS-FtoA | For overexpression of 52K-GFP-KS-FtoA |
| 237692 | p52K-linkerKS | For overexpression of 52K-linkerKS |
| 237693 | p52K-linkerKS-FtoA | For overexpression of 52K-linkerKS-FtoA |