beta-catenin functions as a molecular adapter for disordered cBAF interactions.
Chan YS, Gao Q, Robinson SA, Wang W, Filandrova R, Weinhold LM, Cabrera ML, Zhang M, Ambati CSR, Lerario AM, Putluri N, Kiseljak-Vassiliades K, Wierman ME, Habra MA, Hammer GD, Veverka V, Cermakova K, Hodges HC
Mol Cell. 2025 Jul 15:S1097-2765(25)00576-3. doi: 10.1016/j.molcel.2025.06.026.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 242209 | pPB-CAG-ARID1A-FLAG | Expression of full-length human ARID1A using piggyBac transposition |
| 242210 | pPB-CAG-ARID1A-deltaIDR1-FLAG | Expression of human ARID1A ∆IDR1 (deletion of a.a. 2–970) using piggyBac transposition |
| 242211 | pPB-CAG-ARID1A-deltaIDR1ARID-FLAG | Expression of human ARID1A ∆IDR1 and ∆ARID (deletion of a.a. 2-1169) using piggyBac transposition |
| 242212 | pPB-CAG-ARID1A-CBR-FLAG | Expression of core binding region (CBR) of human ARID1A using piggyBac transposition. ∆IDR1, ∆ARID, and ∆IDR2 (deletion of a.a 2-1610) |
| 242213 | pPB-CAG-ARID1A-deltaIDR1IDR2-FLAG | Expression of human ARID1A ∆IDR1 and ∆IDR2 (deletion of a.a. 2-970 and a.a. 1170-1610) using piggyBac transposition |
| 242214 | pPB-CAG-ARID1A-deltaARIDIDR2-FLAG | Expression of human ARID1A ∆ARID and ∆IDR2 (deletion of a.a. 971-1610) using piggyBac transposition |
| 242215 | pPB-CAG-ARID1A-deltaIDR2-FLAG | Expression of human ARID1A ∆IDR2 (deletion of a.a. 1170-1610) using piggyBac transposition |
| 242216 | pMAL-c4X-MBP-ARID1A-IDR2 | Expression of MBP-tagged human ARID1A IDR2 (a.a. 1170-1610) in E. coli |
| 242217 | pPB-CAG-CTNNB1-HA | Expression of full-length human β-catenin using piggyBac transposition |
| 242218 | pPB-CAG-CTNNB1-deltaN-HA | Expression of human β-catenin ∆N terminal IDR (deletion of a.a. 2-133) using piggyBac transposition |
| 242219 | pPB-CAG-CTNNB1-delta1-3-HA | Expression of human β-catenin ∆arm 1-3 (deletion of a.a. 134-266) using piggyBac transposition |
| 242220 | pPB-CAG-CTNNB1-delta4-6-HA | Expression of human β-catenin ∆arm 4-6 (deletion of a.a. 268-394) using piggyBac transposition |
| 242221 | pPB-CAG-CTNNB1-delta7-9-HA | Expression of human β-catenin ∆arm 7-9 (deletion of a.a. 395-520) using piggyBac transposition |
| 242222 | pPB-CAG-CTNNB1-delta10-12-HA | Expression of human β-catenin ∆arm 10-12 (deletion of a.a. 522-664) using piggyBac transposition |
| 242223 | pPB-CAG-CTNNB1-deltaC-HA | Expression of human β-catenin ∆C terminal IDR (deletion of a.a. 665-781) using piggyBac transposition |
| 242224 | pPB-CAG-CTNNB1-Armadillo-HA | Expression of human β-catenin Armadillo repeats (deletion of a.a. 2-133 and a.a. 665-781) using piggyBac transposition |
| 242225 | pGEX-5X-1-GST-Armadillo | Expression of GST-tagged human β-catenin Armadillo repeats without IDRs (deletion of a.a. 2-133 and 672-781) in E. coli |
| 242226 | pGEX-5X-1-GST-Armadillo-delta4-6 | Expression of GST-tagged human β-catenin Armadillo repeats ∆4-6 without IDRs (deletion of a.a. 2-133, 268-394, and 672-781) in E. coli |
| 242227 | pPB-CAG-NR5A1-FLAG | Expression of full-length human SF-1 using piggyBac transposition |
| 242228 | pPB-CAG-NR5A1-deltaLBD-FLAG | Expression of human SF-1 ∆LBD (deletion of a.a. 221-459) using piggyBac transposition |
| 242229 | pRRL-CAG-NR5A1-FLAG | Expression of full-length human SF-1 using lentivirus |