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Cerebral blood flow is modulated by astrocytic cAMP elevation independently of IP(3)R2-mediated Ca(2+) signaling in mice.

Vittani M, Herlo R, Wang X, Christensen MDB, Vo CT, Mishima T, Kusk P, Konno A, Hirai H, Tsuboi T, Kitaguchi T, Kjaerby C, Asiminas A, Yokoyama T, Sakamoto M, Nedergaard M, Hirase H
Proc Natl Acad Sci U S A. 2025 Jul 8;122(27):e2422069122. doi: 10.1073/pnas.2422069122. Epub 2025 Jul 1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
228394pAAV-mGFAP-Lck-PinkFlamindoAAV vector to express the red cAMP indicator in astrocytes
234548pAAV-mGFAP(ABC1D)-2pabPAC(F198Y)to express a version with reduced constitutive activity (thanks to point mutation F198Y) of the optogenetic tool 2pabPAC, which increases cAMP levels when stimulated by blue light, in astrocytes

Antibodies from Article