Cerebral blood flow is modulated by astrocytic cAMP elevation independently of IP(3)R2-mediated Ca(2+) signaling in mice.
Vittani M, Herlo R, Wang X, Christensen MDB, Vo CT, Mishima T, Kusk P, Konno A, Hirai H, Tsuboi T, Kitaguchi T, Kjaerby C, Asiminas A, Yokoyama T, Sakamoto M, Nedergaard M, Hirase H
Proc Natl Acad Sci U S A. 2025 Jul 8;122(27):e2422069122. doi: 10.1073/pnas.2422069122. Epub 2025 Jul 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228394 | pAAV-mGFAP-Lck-PinkFlamindo | AAV vector to express the red cAMP indicator in astrocytes |
| 234547 | pAAV-mGFAP(ABC1D)-2pabPAC | to express the optogenetic tool 2pabPAC, which increases cAMP levels when stimulated by blue light, in astrocytes |
| 234548 | pAAV-mGFAP(ABC1D)-2pabPAC(F198Y) | to express a version with reduced constitutive activity (thanks to point mutation F198Y) of the optogenetic tool 2pabPAC, which increases cAMP levels when stimulated by blue light, in astrocytes |