A modular cell-free protein biosensor platform using split T7 RNA polymerase.
McSweeney MA, Patterson AT, Loeffler K, Cuellar Lelo de Larrea R, McNerney MP, Kane RS, Styczynski MP
Sci Adv. 2025 Feb 21;11(8):eado6280. doi: 10.1126/sciadv.ado6280. Epub 2025 Feb 21.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 216336 | pT7RNAPNev-NB1 | Arabinose-inducible PBad promoter driving expression of the evolved N-terminal portion (residues 1-179) of T7RNAP translationally fused to anti-eGFP nanobody NB1 |