Skip to main content
Addgene

Cap-independent co-expression of dsRNA-sensing and NF-κB pathway inhibitors enables controllable self-amplifying RNA expression with reduced immunotoxicity

Lim TKY, Ritoux A, Paine LW, Ferguson L, Abdul T, Grundy LJ, Smith ESJ
bioRxiv 2024.09.24.614636 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
242407VEE-mScarlet3-IRES-EGFP-IRES-moxBFPNative (conventional) self-amplifying RNA (saRNA) control construct expresses moxBFP as visual marker of IRES functionality
242408VEE-mScarlet3-IRES-EGFP-IRES-E3Self-amplifying RNA (saRNA) E3 construct; expresses the vaccinia virus E3 protein, which binds and sequesters dsRNA broadly inhibiting dsRNA-sensing pathways
242409VEE-mScarlet3-IRES-EGFP-IRES-E3-NSs-L*Self-amplifying RNA (saRNA) E3-NSs-L* construct; co-expresses vaccinia virus E3, Toscana virus NSs, and Theiler’s virus L*; more comprehensive inhibition of dsRNA-sensing pathways than plasmid 242408.
242413VEE-moxBFP-IRES-PuroRSpectral unmixing: moxBFP only
242414VEE-EGFP-IRES-PuroRSpectral unmixing: EGFP only
242415VEE-mScarlet3-IRES-PuroRSpectral unmixing: mScarlet3 only

Antibodies from Article