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Sequence-Independent RNA Sensing in Living Mammalian Cells

Kolber NS, K. Kaseniit E, Lanz TV, Robinson WH, Gao XJ
bioRxiv 2025.09.18.677213 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
247268EK0574 CAG-ADAR1-p150Expresses human ADAR1-p150 from the CAG promoter
247269EK0656 SFFV-mCherry-SGc(70587.os4)cSG-EGFP-(3xMS2)modulADAR sensor against sythetic target transcript (syn GluR-B stemloop)
247270EK0698 SFFV-mCherry-SGc(s70587.os13)cSG-EGFP-(3xMS2) (FLP-IN)modulADAR sensor against sythetic target transcript (45ntGABRA3 stemloop)
247271EK0716 SFFV-mCherry-SGc(s70587.os18)cSG-EGFP-(3xMS2) (FLP-IN)modulADAR sensor against sythetic target transcript (35nt GluR-B stemloop)
247272EK0717 SFFV-mCherry-SGc(s70587.os19)cSG-EGFP-(3xMS2) (FLP-IN)modulADAR sensor against sythetic target transcript (41nt GluR-B stemloop)
247273EK0718 SFFV-mCherry-SGc(s70587.os20)cSG-EGFP-(3xMS2) (FLP-IN)modulADAR sensor against sythetic target transcript (36nt GABRA3 stem-loop)
247275NSK162 CMV-TO-SMN2 Nluc - inactive (exon 6, 8)Expresses human SMN2 exons 6, 8 followed by inactive Nluc
247277NSK173 SFFV-mCherry-SGc(Smn2ex7.os13-72bp)cSG-EGFP-(3xMS2) (FLP-IN)modulADAR sensor for SMN exon 7
247278NSK176 U6TO EBER1Expreses EBER1
247279NSK177 U6TO EBER2Expresses EBER2
247281NSK195 SFFV-mCherry-SGc(EBER2.os13.9)cSG-EGFP-(3xMS2) (FLP-IN)modulADAR sensor for EBER2
247282NSK241 SFFV-mCherry-SGc(os13.NOT-SMN2.45,55,45)cSG-EGFP-(3xMS2) (FLP-IN)modulADAR sensor for NOT SMN exon 7
247284NSK249 CMV-TO-SCN1A (exons 20, 21)Expresses human SCN1a exons 20, 21
247285NSK250 CMV-TO-SCN1A (exons 20, 20N, 21)Expresses human SCN1a exons 20, 20N, 21
247286NSK252 SFFV-mCherry-SGc(os13.NOT-SCN1A_20N.45,64,45)cSG-EGFP-(3xMS2) (FLP-IN)modulADAR sensor for NOT SCN1a exon 20N
247287NSK266 SFFV-mCherry-SGc(EBER1.linear.2)cSG-EGFP-(3xMS2) (FLP-IN)Linear sensor for EBER1
247288NSK267 SFFV-mCherry-SGc(EBER2.linear.1)cSG-EGFP-(3xMS2) (FLP-IN)Linear sensor for EBER2
247289NSK418 SFFV-mCherry-SGc(s70587.os13.lowbBG.16bp loop)cSG-EGFP-(3xMS2) (FLP-IN)Low baseline modulADAR sensor for synthetic target transcript
247290NSK465 SFFV-mCherry-SGc(os13.NOT-SCN1A_20N.45,64,45.lowBG.12bp loop)cSG-EGFP-(3xMS2) (FLP-IN)Low baseline modulADAR sensor for NOT SCN1a exon 20N
247291NSK477 SFFV-mCherry-SGc(Smn2ex7.os13-72bp.lowBG.16bp loop)cSG-EGFP-(3xMS2) (FLP-IN)Low baseline modulADAR sensor for SMN exon 7

Antibodies from Article