Continuous in situ synthesis of a complete set of tRNAs sustains steady-state translation in a recombinant cell-free system.

Li F, Baranwal AK, Maerkl SJ
Nat Commun. 2025 Jul 5;16(1):6212. doi: 10.1038/s41467-025-61671-8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
242430	pUC19_21 tRNA genes	In vitro transcription of 21 tRNAs after Nt.BspQI treatment. The plasmid contains a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene.
242431	pETA_21 tRNA genes	Expresses 21 tRNAs. The plasmid contains a T7 promoter upstream and an Nt.BspQI recognition site as well as a T7 terminator downstream of each tRNA gene.
242436	pET-15b_T. maritima His-tRNase Z	Expresses T. maritima tRNase Z in E. coli.