Continuous in situ synthesis of a complete set of tRNAs sustains steady-state translation in a recombinant cell-free system.
Li F, Baranwal AK, Maerkl SJ
Nat Commun. 2025 Jul 5;16(1):6212. doi: 10.1038/s41467-025-61671-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 242430 | pUC19_21 tRNA genes | In vitro transcription of 21 tRNAs after Nt.BspQI treatment. The plasmid contains a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene. |
| 242431 | pETA_21 tRNA genes | Expresses 21 tRNAs. The plasmid contains a T7 promoter upstream and an Nt.BspQI recognition site as well as a T7 terminator downstream of each tRNA gene. |