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DASH: a versatile and high-capacity gene stacking system for plant synthetic biology.

Zhao C, Stepanova AN, Alonso JM
Plant Biotechnol J. 2025 Jun 10. doi: 10.1111/pbi.70179. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
242515pDASH-DII-α1Donor II vector in DASH and alpha1 vector in GoldenBraid/DASH, with attPCC and attPTT-FRT
242518pDASH-DII-ω2Donor II vector in DASH and omega2 vector in GoldenBraid/DASH, with attPCC and attPTT-FRT
242520pDASH-DIS-α2Donor I vector in DASH and alpha2 vector in GoldenBraid/DASH, with SacB counter-selection, with attBTT and attBCC-FRT
242525pDASH-AIKAcceptor vector I, accepting Donor I first, with attPTT-FRT
242526pDASH-AIIKAcceptor vector II, accepting Donor II first, with attBCC-FRT

Antibodies from Article