High-throughput discovery of regulatory effector domains in human RNA-binding proteins.
Thurm AR, Finkel Y, Andrews C, Cai XS, Benko C, Bintu L
bioRxiv [Preprint]. 2024 Jul 19:2024.07.19.604317. doi: 10.1101/2024.07.19.604317.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 242611 | pAT031 | Empty MCP-fusion lentiviral construct for generating MCP-tethering RBP libraries in human cells |
| 242612 | pAT039 | 24-stem loop MS2 Citrine + magnetic separation RNA reporter, stem-loops in 5'UTR |
| 242614 | pAT209 | 7-stem loop MS2 Citrine + magnetic separation RNA reporter, stem-loops in 5'UTR |
| 242615 | pAT124 | Degron-tagged MCP construct for generating inducibly stable tethered RBPs |
| 242616 | synNANOS | TMP-inducible strong RNA regulator (tethered to MCP) |