Benjamin Kleinstiver Lab: Kleinstiver paper in preparation 2025
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 242651 | pCMV-ABE8.8m-WT-P2A-EGFP (BKS953) | CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with SpCas9(D10A) and P2A-EGFP |
| 242652 | pCMV-ABE8.8m-SpG-P2A-EGFP (BKS965) | CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with SpG(D10A) and P2A-EGFP |
| 242653 | pCMV-ABE8.8m-VRQR-P2A-EGFP (BKS971) | CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with nVRQR(D10A) and P2A-EGFP |
| 242654 | pCMV-ABEmax-eVRQR-P2A-EGFP (LLH575) | CMV promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with neVRQR(D10A) and P2A-EGFP |
| 242655 | pCMV-ABE8.8m-eVRQR-P2A-EGFP (BKS974) | CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with neVRQR(D10A) and P2A-EGFP |
| 242656 | pCMV-ABE8.20m-eVRQR-P2A-EGFP (LLH569) | CMV promoter expression plasmid for human codon optimized ABE8.20 A-to-G base editor with neVRQR(D10A) and P2A-EGFP |
| 242657 | pCMV-ABE8e-eVRQR-P2A-EGFP (HES1425) | CMV promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with neVRQR(D10A) and P2A-EGFP |
| 242659 | N-term AAV-ABE8e-SpCas9(S55R) (CA21) | CBh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-SpCas9(S55R) - to be used with eVRQR constructs |
| 242660 | C-term AAV-SpCas9-VRQR-gRNA_entry (HES1477) | CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and pU6 SpCas9 gRNA entry cassette |
| 242661 | C-term AAV-eVRQR-ACTA2-gRNA-A4 (human) (LLH301) | CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and human gRNA-A4 |
| 242662 | C-term AAV-eVRQR-ACTA2-gRNA-A4 (mouse) (LLH310) | CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and mouse gRNA-A4 |
| 242663 | pCMV-eVRQR-P2A-EGFP (KAC1028) | CMV promoter expression plasmid for human codon optimized SpCas9-eVRQR(S55R)-BPNLS(SV40)-3xFLAG-P2A-EGFP |
| 242664 | pCMV-SpCas9-VQR-P2A-EGFP (LRF199) | CMV promoter expression plasmid for human codon optimized SpCas9-VQR-BPNLS(SV40)-3xFLAG-P2A-EGFP |
| 242665 | pCMV-SpCas9-VRQR(+A61R)-P2A-EGFP (KAC1030) | CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(A61R)-BPNLS(SV40)-3xFLAG-P2A-EGFP |
| 242666 | pCMV-SpCas9-VRQR(+R221K)-P2A-EGFP (LRF95) | CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(R221K)-BPNLS(SV40)-3xFLAG-P2A-EGFP |
| 242667 | pCMV-SpCas9-VRQR(+L244Q)-P2A-EGFP (LRF96) | CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L244Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP |
| 242668 | pCMV-SpCas9-VRQR(+D269A)-P2A-EGFP (LRF64) | CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D269A)-BPNLS(SV40)-3xFLAG-P2A-EGFP |
| 242669 | pCMV-SpCas9-VRQR(+G366R)-P2A-EGFP (KAC1026) | CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(G366R)-BPNLS(SV40)-3xFLAG-P2A-EGFP |
| 242670 | pCMV-SpCas9-VRQR(+N394K)-P2A-EGFP (KAC956) | CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(N394K)-BPNLS(SV40)-3xFLAG-P2A-EGFP |
| 242671 | pCMV-SpCas9-VRQR(+G1104K)-P2A-EGFP (LRF54) | CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(G1140K)-BPNLS(SV40)-3xFLAG-P2A-EGFP |
| 242672 | pCMV-SpCas9-VRQR(+L1111H)-P2A-EGFP (LRF269) | CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1111H)-BPNLS(SV40)-3xFLAG-P2A-EGFP |
| 242674 | pCMV-SpCas9-VRQR(+T1138K)-P2A-EGFP (LRF243) | CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(T1138K)-BPNLS(SV40)-3xFLAG-P2A-EGFP |
| 242675 | pCMV-SpCas9-VRQR(+L1245V)-P2A-EGFP (LRF49) | CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1245V)-BPNLS(SV40)-3xFLAG-P2A-EGFP |
| 242676 | pCMV-SpCas9-VRQR(+A1285K)-P2A-EGFP (KAC1134) | CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(A1285K)-BPNLS(SV40)-3xFLAG-P2A-EGFP |
| 242677 | pCMV-SpCas9-VRQR(+N1286Q)-P2A-EGFP (LRF247) | CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(N1286Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP |
| 242678 | pCMV-SpCas9-VRQR(+N1317R)-P2A-EGFP (LRF53) | CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(N1317R)-BPNLS(SV40)-3xFLAG-P2A-EGFP |
| 242680 | pCMV-SpCas9-VRQR(+D1332K)-P2A-EGFP (KAC966) | CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D1332K)-BPNLS(SV40)-3xFLAG-P2A-EGFP |
| 242681 | pCMV-SpCas9-VRQR(+D1332R)-P2A-EGFP (LRF45) | CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D1332R)-BPNLS(SV40)-3xFLAG-P2A-EGFP |
| 242682 | pCMV-pLenti-ACTA2-R179 (WT) (LLH661) | Plasmid for expression of ACTA2-R179 (wild-type) cDNA from a lentiviral cassette |
| 242683 | pCMV-pLenti-ACTA2-R179H (LLH647) | Plasmid for expression of ACTA2-R179H cDNA from a lentiviral cassette |
| 242684 | pCMV-pLenti-ACTA2-M178V (LLH709) | Plasmid for expression of ACTA2-M178V cDNA from a lentiviral cassette |
| 242685 | pCMV-pLenti-ACTA2-L180V (LLH669) | Plasmid for expression of ACTA2-L180V cDNA from a lentiviral cassette |
| 242686 | pCMV-pLenti-ACTA2-L180M (LLH673) | Plasmid for expression of ACTA2-L180M cDNA from a lentiviral cassette |
| 242687 | pCMV-pLenti-ACTA2-D181G (LLH681) | Plasmid for expression of ACTA2-D181G cDNA from a lentiviral cassette |
| 242688 | pCMV-pLenti-ACTA2-L180L (LLH677) | Plasmid for expression of ACTA2-L180L cDNA from a lentiviral cassette |
| 242689 | pCMV-PE2-SpG (HES824) | Prime editing in mammalian cells with SpG-based PE2 prime editors |