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A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types (preprint).

Ben-Simon Y, Hooper M, Narayan S, Daigle T, Dwivedi D, Way SW, Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya X, Roth JR, Allen S, Ayala A, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D, Bowlus J, Boyer G, Brouner K, Casian B, Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Departee M, Donadio N, Dotson N, Egdorf T, Gabitto M, Garcia J, Gary A, Gasperini M, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, Halterman C, Helback O, Hockemeyer D, Huang C, Huff S, Hunker A, Johansen N, Juneau Z, Kalmbach B, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, Leibly M, Lenz GH, Liang E, Lusk N, Malone J, Mollenkopf T, Morin E, Newman D, Ng L, Ngo K, Omstead V, Oyama A, Pham T, Pom CA, Potekhina L, Ransford S, Rette D, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shulga L, Sigler AR, Siverts LA, Somasundaram S, Stewart K, Szelenyi E, Tieu M, Trader C, van Velthoven CTJ, Walker M, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, Levi BP, Ngai J, Ting J, Tasic B
bioRxiv [Preprint]. 2024 Sep 26:2024.06.10.597244. doi: 10.1101/2024.06.10.597244. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
214552AiP1848 - pAAV-AiE2435m-minBG-SYFP2-WPRE-BGHpAAiE2435m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
214582AiP1923 - pAAV-AiE2455m-minBG-SYFP2-WPRE-BGHpAAiE2455m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
220636AiP1325 - pAAV-AiE2100m_C2-minBG-SYFP2-WPRE-BGHpAAiE2100m_C2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
220649AiP1416 - pAAV-AiE2051m_C2-minBG-SYFP2-WPRE-BGHpAAiE2051m_C2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
220651AiP1418 - pAAV-AiE2052m_C2-minBG-SYFP2-WPRE-BGHpAAiE2052m_C2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
220666AiP1758 - pAAV-AiE2434m-minBG-SYFP2-WPRE-BGHpAAiE2434m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
220703AiP2072 - pAAV-AiE2608m-SYFP2-WPRE-BGHpAAiE2608m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
220710AiP1666 - pAAV-AiE0486m-minBG-IRES2-iCre-HGHpAAiE0486m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells
220718AiP1868 - pAAV-AiE2182m-minBG-iCre-WPRE-BGHpAAiE2182m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells
220719AiP1911 - pAAV-AiE2114m-minBG-iCre-WPRE-BGHpAAiE2114m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells
220720AiP1912 - pAAV-AiE2116m-minBG-iCre-WPRE-BGHpAAiE2116m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells
223969AiP11969 - pAAV-hsA2-AiE0140h-minRho-SYFP2-4X2C-WPRE-HGHpA (Alias: CN1969)AiE0140h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Pvalb+ neurons
223970AiP12017 - pAAV-3xSP10ins-AiE0140h_3xC2-minRho-SYFP2-4X2C-WPRE3-BGHpA (Alias: CN2017)AiE0140h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Pvalb+ neurons
223980AiP12293 - pAAV-3xSP10ins-AiE0219h_3xC2-minRho-SYFP2-8X2C-WPRE3-BGHpA (Alias: CN2293)AiE0219h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neurons
224052AiP14908 - pAAV-AiE0354h-minBG-mScarlet-WPRE3-BGHpA (Alias: CN4908)AiE0354h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neurons
224053AiP14937 - pAAV-AiE1367m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4937)AiE1367m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L2/3_IT neurons (note HPF dentate gyrus)
224060AiP12546 - pAAV-AiE0050m_3xC2-minBG-tdTomato-WPRE3-BGHpA (Alias: CN2546)AiE0050m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L4/5_IT neurons
224061AiP12547 - pAAV-hsA2-AiE0140h-minRho-mTFP1-WPRE3-BGHpA (Alias: CN2547)AiE0140h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Pvalb+ neurons
224062AiP15262 - pAAV-AiE0219h-minBG-mScarlet-WPRE3-BGHpA (Alias: CN5262)AiE0219h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neurons
229934AiP11980 - pAAV-DLX2.0-minRho-dTomato-10aa-H2B-WPRE3-BGHpA (Alias: CN1980)DLX2.0 is an enhancer sequence, designed to drive nuclear dTomato expression in forebrain GABAergic neurons
229935AiP13798 - pAAV-DLX2.0-minBG-SYFP2-P2A-mScarlet-10aa-H2B-WPRE3-BGHpA (Alias: CN3798)DLX2.0 is an enhancer sequence, designed to drive cytosolic SYFP2 and nuclear mScarlet expression in forebrain GABAergic neurons
229938AiP15324 - pAAV-hSyn1-mScarlet-10aa-H2B-WPRE3-BGHpA (Alias: CN5324) AAV vector for pan-neuronal expression of nuclear mScarlet
229939AiP15150 - pAAV-AiE0078h_3xC2-minBG-mTFP1-10aa-H2B-WPRE3-BGHpA (Alias: CN5150)AiE0078h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types
230199AiP12309 - pAAV-AiE0130h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2309)AiE0130h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
230201AiP12310 - pAAV-AiE0131h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2310)AiE0131h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
230626AiP1359 - pAAV-AiE2081m-minBG-SYFP2-4X2C-WPRE3-BGHpAAiE2081m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
230628AiP1361 - pAAV-AiE2085m-minBG-SYFP2-4X2C-WPRE3-BGHpAAiE2085m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
230629AiP1362 - pAAV-AiE2088m-minBG-SYFP2-4X2C-WPRE3-BGHpAAiE2088m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Antibodies from Article