A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types (preprint).
Ben-Simon Y, Hooper M, Narayan S, Daigle T, Dwivedi D, Way SW, Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya X, Roth JR, Allen S, Ayala A, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D, Bowlus J, Boyer G, Brouner K, Casian B, Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Departee M, Donadio N, Dotson N, Egdorf T, Gabitto M, Garcia J, Gary A, Gasperini M, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, Halterman C, Helback O, Hockemeyer D, Huang C, Huff S, Hunker A, Johansen N, Juneau Z, Kalmbach B, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, Leibly M, Lenz GH, Liang E, Lusk N, Malone J, Mollenkopf T, Morin E, Newman D, Ng L, Ngo K, Omstead V, Oyama A, Pham T, Pom CA, Potekhina L, Ransford S, Rette D, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shulga L, Sigler AR, Siverts LA, Somasundaram S, Stewart K, Szelenyi E, Tieu M, Trader C, van Velthoven CTJ, Walker M, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, Levi BP, Ngai J, Ting J, Tasic B
bioRxiv [Preprint]. 2024 Sep 26:2024.06.10.597244. doi: 10.1101/2024.06.10.597244.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214552 | AiP1848 - pAAV-AiE2435m-minBG-SYFP2-WPRE-BGHpA | AiE2435m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells |
| 214582 | AiP1923 - pAAV-AiE2455m-minBG-SYFP2-WPRE-BGHpA | AiE2455m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells |
| 220636 | AiP1325 - pAAV-AiE2100m_C2-minBG-SYFP2-WPRE-BGHpA | AiE2100m_C2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells |
| 220649 | AiP1416 - pAAV-AiE2051m_C2-minBG-SYFP2-WPRE-BGHpA | AiE2051m_C2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells |
| 220651 | AiP1418 - pAAV-AiE2052m_C2-minBG-SYFP2-WPRE-BGHpA | AiE2052m_C2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells |
| 220666 | AiP1758 - pAAV-AiE2434m-minBG-SYFP2-WPRE-BGHpA | AiE2434m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells |
| 220703 | AiP2072 - pAAV-AiE2608m-SYFP2-WPRE-BGHpA | AiE2608m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells |
| 220710 | AiP1666 - pAAV-AiE0486m-minBG-IRES2-iCre-HGHpA | AiE0486m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells |
| 220718 | AiP1868 - pAAV-AiE2182m-minBG-iCre-WPRE-BGHpA | AiE2182m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells |
| 220719 | AiP1911 - pAAV-AiE2114m-minBG-iCre-WPRE-BGHpA | AiE2114m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells |
| 220720 | AiP1912 - pAAV-AiE2116m-minBG-iCre-WPRE-BGHpA | AiE2116m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells |
| 223969 | AiP11969 - pAAV-hsA2-AiE0140h-minRho-SYFP2-4X2C-WPRE-HGHpA (Alias: CN1969) | AiE0140h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Pvalb+ neurons |
| 223970 | AiP12017 - pAAV-3xSP10ins-AiE0140h_3xC2-minRho-SYFP2-4X2C-WPRE3-BGHpA (Alias: CN2017) | AiE0140h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Pvalb+ neurons |
| 223980 | AiP12293 - pAAV-3xSP10ins-AiE0219h_3xC2-minRho-SYFP2-8X2C-WPRE3-BGHpA (Alias: CN2293) | AiE0219h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neurons |
| 224052 | AiP14908 - pAAV-AiE0354h-minBG-mScarlet-WPRE3-BGHpA (Alias: CN4908) | AiE0354h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neurons |
| 224053 | AiP14937 - pAAV-AiE1367m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4937) | AiE1367m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L2/3_IT neurons (note HPF dentate gyrus) |
| 224060 | AiP12546 - pAAV-AiE0050m_3xC2-minBG-tdTomato-WPRE3-BGHpA (Alias: CN2546) | AiE0050m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L4/5_IT neurons |
| 224061 | AiP12547 - pAAV-hsA2-AiE0140h-minRho-mTFP1-WPRE3-BGHpA (Alias: CN2547) | AiE0140h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Pvalb+ neurons |
| 224062 | AiP15262 - pAAV-AiE0219h-minBG-mScarlet-WPRE3-BGHpA (Alias: CN5262) | AiE0219h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neurons |
| 229934 | AiP11980 - pAAV-DLX2.0-minRho-dTomato-10aa-H2B-WPRE3-BGHpA (Alias: CN1980) | DLX2.0 is an enhancer sequence, designed to drive nuclear dTomato expression in forebrain GABAergic neurons |
| 229935 | AiP13798 - pAAV-DLX2.0-minBG-SYFP2-P2A-mScarlet-10aa-H2B-WPRE3-BGHpA (Alias: CN3798) | DLX2.0 is an enhancer sequence, designed to drive cytosolic SYFP2 and nuclear mScarlet expression in forebrain GABAergic neurons |
| 229937 | AiP14019 - pAAV-hSyn1-mTFP1-10aa-H2B-WPRE3-BGHpA (Alias: CN4019) | AAV vector for pan-neuronal expression of nuclear mTFP1 |
| 229938 | AiP15324 - pAAV-hSyn1-mScarlet-10aa-H2B-WPRE3-BGHpA (Alias: CN5324) | AAV vector for pan-neuronal expression of nuclear mScarlet |
| 229939 | AiP15150 - pAAV-AiE0078h_3xC2-minBG-mTFP1-10aa-H2B-WPRE3-BGHpA (Alias: CN5150) | AiE0078h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types |
| 230199 | AiP12309 - pAAV-AiE0130h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2309) | AiE0130h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells |
| 230201 | AiP12310 - pAAV-AiE0131h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2310) | AiE0131h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells |
| 230626 | AiP1359 - pAAV-AiE2081m-minBG-SYFP2-4X2C-WPRE3-BGHpA | AiE2081m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells |
| 230628 | AiP1361 - pAAV-AiE2085m-minBG-SYFP2-4X2C-WPRE3-BGHpA | AiE2085m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells |
| 230629 | AiP1362 - pAAV-AiE2088m-minBG-SYFP2-4X2C-WPRE3-BGHpA | AiE2088m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells |