Nickase fidelity drives EvolvR-mediated diversification in mammalian cells.
Hurtado JE, Schieferecke AJ, Halperin SO, Guan J, Aidlen D, Schaffer DV, Dueber JE
Nat Commun. 2025 Apr 19;16(1):3723. doi: 10.1038/s41467-025-58414-0.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 249060 | pEvolvR-mCherry-enCas9(D10A)-PolI5M | Expresses nucleus-localized enCas9 (D10A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs. |
| 249061 | pEvolvR-mCherry-enCas9(D10A)-PolI3M | Expresses nucleus-localized enCas9 (D10A) fused to PolI3M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs. |
| 249063 | p-mCherry-enCas9(D10A) | Expresses nucleus-localized enCas9 (D10A) fused to mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs. |
| 249064 | pEvolvR-mCherry-enCas9(H840A)-PolI5M | Expresses nucleus-localized enCas9 (H840A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs. |
| 249065 | p-mCherry-dCas9-PolI5M | Expresses nucleus-localized dCas9 fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs. |
| 249066 | pEvolvR-mCherry-nCas9-PolI5M | Expresses nucleus-localized nCas9 (D10A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs. |
| 249067 | p-mCherry | Expresses nucleus-localized mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs. |
| 249068 | pEvolvR-mCherry-enCas9(D10A)-PolI5MΔ | Expresses nucleus-localized enCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs. |
| 249069 | pEvolvR-nCas9(D10A)-PolI5MΔ | Expresses nucleus-localized nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs. |
| 249070 | pEvolvR-mCherry-SuperFi-nCas9(D10A)-PolI5MΔ | Expresses nucleus-localized SuperFi-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs. |
| 249071 | pEvolvR-mCherry-Sniper-nCas9(D10A)-PolI5MΔ | Expresses nucleus-localized Sniper-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs. |
| 249072 | pEvolvR-mCherry-LZ3-nCas9(D10A)-PolI5MΔ | Expresses nucleus-localized LZ3-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs. |
| 249073 | pEvolvR-mCherry-HSC1.2-nCas9(D10A)-PolI5MΔ | Expresses nucleus-localized HSC1.2-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs. |
| 249074 | pEvolvR-mCherry-SpRY-nCas9(D10A)-PolI5MΔ | Expresses nucleus-localized SpRY-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs. |
| 249075 | pEvolvR-mCherry-HF1-nCas9(D10A)-PolI5MΔ | Expresses nucleus-localized HF1-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs. |
| 249076 | pEvolvR-mCherry-Hypa-nCas9(D10A)-PolI5MΔ | Expresses nucleus-localized Hypa-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs. |
| 249077 | pEvolvR-mCherry-eNme2.C.NR-nCas9(D16A)-PolI5MΔ | Expresses nucleus-localized eNme2.C.NR-nCas9 (D16A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs. |
| 249078 | pEvolvR-mCherry-Slug-nCas9(D10A)-PolI5MΔ | Expresses nucleus-localized Slug-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs. |
| 249079 | pEvolvR-mCherry-NNG-Slug-nCas9(D10A)-PolI5MΔ | Expresses nucleus-localized NNG-Slug-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs. |