Nickase fidelity drives EvolvR-mediated diversification in mammalian cells.
Hurtado JE, Schieferecke AJ, Halperin SO, Guan J, Aidlen D, Schaffer DV, Dueber JE
Nat Commun. 2025 Apr 19;16(1):3723. doi: 10.1038/s41467-025-58414-0.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 249061 | pEvolvR-mCherry-enCas9(D10A)-PolI3M | Expresses nucleus-localized enCas9 (D10A) fused to PolI3M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs. |
| 249063 | p-mCherry-enCas9(D10A) | Expresses nucleus-localized enCas9 (D10A) fused to mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs. |
| 249064 | pEvolvR-mCherry-enCas9(H840A)-PolI5M | Expresses nucleus-localized enCas9 (H840A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs. |
| 249065 | p-mCherry-dCas9-PolI5M | Expresses nucleus-localized dCas9 fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs. |
| 249067 | p-mCherry | Expresses nucleus-localized mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs. |