Synthetic type III-E CRISPR-Cas effectors for programmable RNA-targeting
Brogan DJ, Dalla Benetta E, Wang T, Lin CP, Chen F, Li H, Lin C, Komives EA, Akbari OS
bioRxiv 2024.02.23.581838; doi: https://doi.org/10.1101/2024.02.23.581838
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 208589 | CMV-SynCas.v10 mammalian expression | Expression of SynCas.v10 for analysis of RNA knockdown |
| 208590 | CMV-SynCas.v14A mammalian expression | Expression of SynCas.v14A for analysis of RNA knockdown |
| 208591 | CMV-SynCas.v35 mammalian expression | Expression of SynCas.v35 for analysis of RNA knockdown |
| 208592 | CMV-SynCas.v39 mammalian expression | Expression of SynCas.v39 for analysis of RNA knockdown |
| 208593 | CMV-SynCas.v41 mammalian expression | Expression of SynCas.v41 for analysis of RNA knockdown |
| 208594 | T7-RBS-6xHis-SUMO-SynCas.v10 bacteria | Production and purification of catalytically active SynCas.v10 |
| 208595 | T7-RBS-6xHis-SUMO-dSynCas.v10 bacteria | Production and purification of catalytically inactive SynCas.v10 |
| 208596 | T7-RBS-6xHis-SUMO-SynCas.v12 bacteria | Production and purification of catalytically active SynCas.v12 |
| 208597 | T7-RBS-6xHis-SUMO-dSynCas.v12 bacteria | Production and purification of catalytically inactive SynCas.v12 |
| 209003 | CMV-SynCas.v47 mammalian expression | Expression of SynCas.v47 for analysis of RNA knockdown |