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Mutation of an active site-adjacent residue in VIM indirectly dictates interactions with and blunts inhibition by D-captopril.

Silwal SB, Wamsley B, Wang Z, Gung BW, Nix JC, Page RC
J Inorg Biochem. 2025 Oct;271:112975. doi: 10.1016/j.jinorgbio.2025.112975. Epub 2025 Jun 10. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
242840pET-28a_VIM-2Expresses His6-TEV-VIM-2 in bacteria, comprising a His6-tag, the Tobacco etch virus (TEV) cleavage site “ENLYFQ/G", and the VIM-2 sequence (UniPROT Q5U7L7)
242841pET-28a_VIM-15Expresses His6-TEV-VIM-15 in bacteria, comprising a His6-tag, the Tobacco etch virus (TEV) cleavage site “ENLYFQ/G", and the VIM-15 sequence (UniPROT B4YAJ6)
242842pET-28a_VIM-20Expresses His6-TEV-VIM-20 in bacteria, comprising a His6-tag, the Tobacco etch virus (TEV) cleavage site “ENLYFQ/G", and the VIM-20 sequence (UniPROT A0A344X7M2)
242843pET-28a_VIM-31Expresses His6-TEV-VIM-31 in bacteria, comprising a His6-tag, the Tobacco etch virus (TEV) cleavage site “ENLYFQ/G", and the VIM-31 sequence (UniPROT I3RJZ3)

Antibodies from Article