Systematic Mapping of Bacterial CRISPRa Systems for Synergistic Gene Activation Reveals Antagonistic Effects.
Kiattisewee C, Karanjia AV, Cardiff RAL, Olander KE, Leejareon P, Alvi SS, Carothers JM, Zalatan JG
ACS Synth Biol. 2025 Jul 22. doi: 10.1021/acssynbio.5c00358.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 242906 | pCK320 | aTc-inducible expression of dCas9-AsiA |
| 242907 | pCK071 | Constitutive expression of dCas9 and PspF-λN22 |
| 242908 | pCK157 | Constitutive expression of dCas9, MCP-SYNZIP6, and αNTD-SYNZIP5 |
| 242909 | pCK147.02 | Constitutive expression of dCas9, PspF-λN22, and boxB scRNA J102 |
| 242910 | pCK692 | Constitutive expression of dCas9 and αNTD-MCP-SoxS |
| 242912 | pCK321 | aTc-inducible expression of dCas9-AsiA and constitutive expression of MCP-SoxS |
| 242913 | pKO025 | aTc-inducible expression of Spy-dCas9-AsiA and Sth1-dCas9, and constitutive expression of MCP-SoxS |
| 242914 | pCK660 | Constitutive expression of dCas9, MCP-SoxS, and PspF-λN22 |
| 242916 | pCK761 | Constitutive expression of dCas9, αNTD-MCP, and PspF-λN22 |
| 242917 | pCK948 | Constitutive expression of dCas9, MCP-TetD, and PspF-λN22 |
| 242920 | pAK011 | Reporter J1-J3(122) -BBa_J23117-mRFP |
| 242923 | pKO023 | Reporter J1-J3(122)-BBa_J23117 with Sth1 PAM |