Protocol combining RNA interference and regeneration assays in planarian embryos.
Deihl EW, Booth CLT, Davies EL
bioRxiv [Preprint]. 2025 Jul 22:2025.07.21.666018. doi: 10.1101/2025.07.21.666018.
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PubMed
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 242942 | pDL1-Spol-Ovo-Nterm | Encodes an N-terminal fragment of Spol-Ovo for use in generating dsRNA for RNAi in the planarian S. polychroa |
| 242943 | pDL1-Spol-Ovo-C-term | Encodes a C-terminal fragment of Spol-Ovo for use in generating dsRNA for RNAi in the planarian S. polychroa |
| 242944 | pDL1-Spol-foxA-C-term | Encodes a C-terminal fragment of Spol-foxA to generate dsRNA for RNAi experiments in the planarian S. polychroa |
| 242945 | pDL1-eGFP | Encodes a fragment of eGFP for generating dsRNA for use as a control in RNAi experiments |