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Efficient production of gene-edited onion (Allium cepa) plants using biolistic delivery of cas9 RNPs and transient expression constructs.

De La Mora CL, Havey MJ, Krysan PJ
Plant Cell Rep. 2025 Oct 19;44(11):243. doi: 10.1007/s00299-025-03626-3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
243674pStayGoldExpresses StayGold fluorescent protein in plants using the Cestrum Yellow Leaf Curling Virus (CmYLCV) 9.11 promoter and an Arabidopsis HSP terminator
243675pBBMExpresses a maize optimized CDS of BABY BOOM in plants using the Cestrum Yellow Leaf Curling Virus (CmYLCV) 9.11 promoter and an Arabidopsis HSP terminator
243676pWUS2Expresses a maize optimized CDS of WUSCHEL2 in plants using the Cestrum Yellow Leaf Curling Virus (CmYLCV) 9.11 promoter and an Arabidopsis HSP terminator
243677pGRF-GIFExpresses a chimeric protein composed of the wheat GROWTH-REGULATING-FACTOR and its wheat cofactor GRF-INTERACTING-FACTOR1 in plants via the CmYLCV 9.11 promoter and an Arabidopsis HSP terminator
243678pHYGExpresses the HYGROMYCIN PHOSPHOTRANSFERASE (HPT) CDS in plants using the Cestrum Yellow Leaf Curling Virus (CmYLCV) 9.11 promoter and an Arabidopsis HSP terminator

Antibodies from Article