Proximity-specific ribosome profiling reveals the logic of localized mitochondrial translation.
Luo J, Khandwala S, Hu J, Lee SY, Hickey KL, Levine ZG, Harper JW, Ting AY, Weissman JS
Cell. 2025 Aug 25:S0092-8674(25)00916-X. doi: 10.1016/j.cell.2025.08.002.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 243683 | pJL95 | Stably expresses ER localized LOV-BirA |
| 243685 | pJL101 | Stably expresses LOV-BirA-GFP-OMP25 TMD fusion proteins, localized to mitochondrial outer membrane |
| 243686 | pJL163 | Stably expresses ALDH18A1 CDS with its endogenous 5'UTR and 3'UTR |
| 243687 | pJL178 | Stably expresses fusion of SUPV3L1(1-114aa) and ALDH18A1(106-795aa) |
| 243688 | pJL179 | Stably expresses SUPV3L1 CDS, flanked with endogenous 5' and 3'UTRs of ALDH18A1 |
| 243689 | pJL181 | Stably expresses ALDH18A1 CDS (106aa-795aa) with its endogenous 5'UTR and 3'UTR |
| 243690 | pJL191 | Stably expresses a chimeric protein, SUPV3L1(1-199aa)-ALDH18A1(106aa-385aa)-SUPV3L1(451-786aa) |
| 243691 | pJL192 | Stably expresses a chimeric protein, SUPV3L1(1-114aa)-ALDH18A1(106aa-385aa)-SUPV3L1(351-786aa) |
| 243692 | pJL209 | Stably expresses a mitochondrial matrix-targeted mNeonGreen2 (1-10) protein |
| 243693 | pJL233 | Stably expresses a chimeric protein, ALDH18A1(1-97aa)-SUPV3L1 (108aa-358aa)-ALDH18A1 (351-795aa) |
| 243694 | pJL239 | A piggyBac vector containing the NDUFB9 genomic sequence, modified with silent mutations to allow for PCR-based analysis |
| 243695 | pJL240 | A piggyBac vector containing the COX5B genomic sequence, modified with silent mutations to allow for PCR-based analysis |
| 243696 | pJL257 | A piggyBac vector containing the NDUFB9 coding sequences, modified with silent mutations to allow for PCR-based analysis, along with endogenous promoter and UTRs. |
| 243697 | pJL258 | A piggyBac vector containing the endogenous NDUFB9 genomic locus, with its native 5' UTR replaced by the Actb 5' UTR, modified with silent mutations to allow for PCR-based analysis |
| 243698 | pJL259 | A piggyBac vector containing the endogenous NDUFB9 genomic locus, with its native 3' UTR replaced by the Actb 3' UTR, modified with silent mutations to allow for PCR-based analysis |
| 243699 | pJL263 | A piggyBac vector containing the NDUFB9 genomic sequence fused with a mNeonGreen2(11) sequence, modified with silent mutations to allow for PCR-based analysis |
| 243700 | pJL296 | A piggyBac vector containing a synthetic reporter with mNeonGreen2(11) |
| 243702 | pJL304 | A piggyBac vector containing the NDUFB9 coding sequences, without a mitochondrial targeting sequence |
| 243703 | pJL306 | A piggyBac vector containing the NDUFB9 coding sequence (CDS) flanked by the 5' and 3' UTRs of the Actb gene |
| 243704 | pJL308 | A piggyBac vector containing the NDUFB9 coding sequence under the control of its endogenous promoter and 5' UTR, followed by the ACTB 3' UTR |
| 243705 | pJL309 | A piggyBac vector encoding an NDUFB9-mNeonGreen2(11) fusion protein that lacks a mitochondrial targeting sequence. |
| 243706 | pJL345 | A piggyBac vector containing the UQCRQ genomic sequence, modified with silent mutations to allow for PCR-based analysis |
| 243707 | pJL347 | A piggyBac vector containing the COX5B coding sequences, modified with silent mutations to allow for PCR-based analysis, along with endogenous promoter and UTRs. |
| 243708 | pJL349 | Stably expresses a Myc-tagged wild-type AKAP1 containing gRNA-resistant silent mutations |
| 243709 | pJL354 | A piggyBac vector containing the endogenous COX7A2 genomic locus, with its native 5' UTR replaced by the Actb 5' UTR, modified with silent mutations to allow for PCR-based analysis |
| 243710 | pJL355 | A piggyBac vector containing the endogenous COX7A2 genomic locus, with its native 3' UTR replaced by the Actb 3' UTR, modified with silent mutations to allow for PCR-based analysis |
| 243711 | pJL357 | A piggyBac vector containing the COX7A2 coding sequences, modified with silent mutations to allow for PCR-based analysis, along with endogenous promoter and UTRs. |
| 243712 | pJL358 | A piggyBac vector containing the COX7A2 coding sequence (CDS) flanked by the 5' UTR of the COX7A2 gene and 3' UTR of the ACTB gene |
| 243713 | pJL359 | A piggyBac vector containing the COX7A2 coding sequence (CDS) flanked by the 5' and 3' UTRs of the ACTB gene |
| 243714 | pJL361 | A piggyBac vector containing a synthetic reporter containing the 5' and 3' UTRs of COX7A2 flanking a partial mCherry sequence with an intron |
| 243715 | pJL373 | A piggyBac vector containing the COX7A2 genomic sequence, modified with silent mutations to allow for PCR-based analysis |
| 243716 | pJL378 | A piggyBac vector containing the UQCRQ coding sequences, modified with silent mutations to allow for PCR-based analysis, along with endogenous promoter and UTRs. |
| 243717 | pJL388 | Stably expresses a Myc-tagged AKAP1 with a GDDG mutation and gRNA-resistant silent mutations |
| 243718 | pJL394 | Stably expresses a Myc-tagged AKAP1 with tudor domain deleted and gRNA-resistant silent mutations |
| 243719 | pJL396 | Stably expresses a Myc-tagged AKAP1 with PKA helix deleted and gRNA-resistant silent mutations |
| 243720 | pJL397 | Stably expresses a Myc-tagged mcherry |
| 243721 | pJL403 | Stably expresses a chimeric reporter with a mCherry (1-105aa)-XTEN250 linker-TBRG4(351aa-631aa), flanked by 5' and 3'UTRs of ALDH18A1 and no mitochondrial targeting sequence |
| 243722 | pJH061 | Expresses a chimeric form of ALDH18A1, where the region between residue 97 and 351 is replaced by a 250-residue XTEN linker |
| 243723 | pJH063 | Expresses a chimeric form of SUPV3L1, where the region between residue 114 and 351 is replaced by a 250-residue XTEN linker |
| 243724 | pJH101 | Expresses TBRG4 |
| 243725 | pJH102 | Expresses a chimeric form of TBRG4, where the region between residue 110 and 351 is replaced by a 250-residue XTEN linker |
| 243726 | pJH105 | Expresses PMPCA |
| 243727 | pJH106 | Expresses a chimeric form of PMPCA, where the region between residue 100 and 351 is replaced by a 250-residue XTEN linker |
| 243728 | pJH122 | Expresses a fusion between residue 1-97 of ALDH18A1 and a 700-residue XTEN linker |