Proximity-specific ribosome profiling reveals the logic of localized mitochondrial translation.
Luo J, Khandwala S, Hu J, Lee SY, Hickey KL, Levine ZG, Harper JW, Ting AY, Weissman JS
Cell. 2025 Aug 25:S0092-8674(25)00916-X. doi: 10.1016/j.cell.2025.08.002.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 243683 | pJL95 | Stably expresses ER localized LOV-BirA |
| 243685 | pJL101 | Stably expresses LOV-BirA-GFP-OMP25 TMD fusion proteins, localized to mitochondrial outer membrane |
| 243687 | pJL178 | Stably expresses fusion of SUPV3L1(1-114aa) and ALDH18A1(106-795aa) |
| 243688 | pJL179 | Stably expresses SUPV3L1 CDS, flanked with endogenous 5' and 3'UTRs of ALDH18A1 |
| 243690 | pJL191 | Stably expresses a chimeric protein, SUPV3L1(1-199aa)-ALDH18A1(106aa-385aa)-SUPV3L1(451-786aa) |
| 243692 | pJL209 | Stably expresses a mitochondrial matrix-targeted mNeonGreen2 (1-10) protein |
| 243700 | pJL296 | A piggyBac vector containing a synthetic reporter with mNeonGreen2(11) |
| 243703 | pJL306 | A piggyBac vector containing the NDUFB9 coding sequence (CDS) flanked by the 5' and 3' UTRs of the Actb gene |
| 243704 | pJL308 | A piggyBac vector containing the NDUFB9 coding sequence under the control of its endogenous promoter and 5' UTR, followed by the ACTB 3' UTR |
| 243708 | pJL349 | Stably expresses a Myc-tagged wild-type AKAP1 containing gRNA-resistant silent mutations |
| 243717 | pJL388 | Stably expresses a Myc-tagged AKAP1 with a GDDG mutation and gRNA-resistant silent mutations |
| 243718 | pJL394 | Stably expresses a Myc-tagged AKAP1 with tudor domain deleted and gRNA-resistant silent mutations |
| 243719 | pJL396 | Stably expresses a Myc-tagged AKAP1 with PKA helix deleted and gRNA-resistant silent mutations |
| 243720 | pJL397 | Stably expresses a Myc-tagged mcherry |
| 243721 | pJL403 | Stably expresses a chimeric reporter with a mCherry (1-105aa)-XTEN250 linker-TBRG4(351aa-631aa), flanked by 5' and 3'UTRs of ALDH18A1 and no mitochondrial targeting sequence |
| 243722 | pJH061 | Expresses a chimeric form of ALDH18A1, where the region between residue 97 and 351 is replaced by a 250-residue XTEN linker |
| 243723 | pJH063 | Expresses a chimeric form of SUPV3L1, where the region between residue 114 and 351 is replaced by a 250-residue XTEN linker |
| 243724 | pJH101 | Expresses TBRG4 |
| 243725 | pJH102 | Expresses a chimeric form of TBRG4, where the region between residue 110 and 351 is replaced by a 250-residue XTEN linker |
| 243726 | pJH105 | Expresses PMPCA |
| 243727 | pJH106 | Expresses a chimeric form of PMPCA, where the region between residue 100 and 351 is replaced by a 250-residue XTEN linker |