Targeted delivery of diverse biomolecules with engineered bacterial nanosyringes.
Kreitz J, Yang V, Friedrich MJ, Pham J, Macrae RK, Zhang F
Nat Biotechnol. 2025 Aug 12. doi: 10.1038/s41587-025-02774-x.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 243733 | pAWP78-PVCpnf_pvc13-Ad5_pvc10-Cas9 | PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with Cas9 on CTD |
| 243735 | pAWP78-PVCpnf_pvc13-Ad5_Cas9-Pvc10 | PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with Cas9 on NTD |
| 243737 | pAWP78-PVCpnf_pvc13-Ad5_pvc8-Cas9 | PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike base (Pvc8) loaded with Cas9 on CTD |
| 243738 | pAWP78-PVCpnf_pvc12-FLAG_Δpvc10 | PVC structural operon - FLAG-tagged baseplate (Pvc12) - deleted spike tip (Pvc10) |
| 243739 | pAWP78-PVCpnf_pvc13-SpyTag | PVC structural operon - tail fiber (Pvc13) tagged with SpyTag |
| 243740 | pAWP78-PVCpnf_pvc13-SpyTag_Δpvc10 | PVC structural operon - tail fiber (Pvc13) tagged with SpyTag - deleted spike tip (Pvc10) |
| 243741 | pAWP78-PVCpnf_pvc13-SNAP | PVC structural operon - tail fiber (Pvc13) tagged with SNAP tag |
| 243742 | pAWP78-PVCpnf_pvc13-Ad5_Δpvc10 | PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - deleted spike tip (Pvc10) |
| 243743 | pAWP78-PVCpnf_pvc13-Ad5_pvc10-ZFD | PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with C half of a zinc finger deaminase (ZFD) on CTD |
| 243744 | pAWP78-PVCpnf_pvc13-Ad5_HUHe-Pvc10-Cas9 | PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with HUHe on NTD and Cas9 on CTD |
| 243745 | pAWP78-PVCpnf_pvc13-Ad5_pvc10-HUHe | PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with HUHe on CTD |
| 243747 | pBR322-sgRNA-pvc18-20 | PVC cargo/regulatory region - deleted toxin cargos (Pdp1/Pnf) - vegfa sgRNA |
| 243748 | pBR322-sgRNA-pvc18-20-Pdp1_NTD-Cre | PVC cargo/regulatory region - deleted toxin cargos (Pdp1/Pnf) - vegfa sgRNA - Cre loaded into the PVC tube via Pdp1 (NTD) |
| 243750 | TwinStrepSUMO-pvc10 | For affinity purification of spike tip (Pvc10) |
| 243751 | TwinStrepSUMO-Ad5-SpyCatcher | For affinity purification of A5 knob fused to SpyCatcher |
| 243752 | TwinStrepSUMO-Nb-SpyCatcher | For affinity purification of anti-mouse MHC-II nanobody (Nb) fused to SpyCatcher |
| 243753 | TwinStrepSUMO-scFv-SpyCatcher | For affinity purification of anti-HA scFv fused to SpyCatcher |
| 243754 | TwinStrepSUMO-DARPin-SpyCatcher | For affinity purification of E01 anti-EGFR DARPin fused to SpyCatcher |
| 243755 | TwinStrepSUMO-pvc10-Cas9 | For affinity purification of Pvc10 fused to Cas9 |