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Live-cell Imaging of Endocytic Transport using Functionalized Nanobodies in Cultured Cells

Buser DP, Schleicher KD, Junne T, Biehlmaier O
Jove Journal 2025 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
243765pET24a-VHH-mNeptune2.5Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mNeptune2.5. VHH-mNeptune2.5 also contains a T7, HA, BAP and His6 epitope
243766pET24a-VHH-mCardinal2Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCardinal2. VHH-mCardinal2 also contains a T7, HA, BAP and His6 epitope
243768pET24a-VHH-iRFP670Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to iRFP670. VHH-iRFP670 also contains a T7, HA, BAP and His6 epitope

Antibodies from Article