Boolean logic-gated protein presentation through autonomously compiled molecular topology.

Gharios R, Ross ML, Li A, Kottantharayil SP, Hoye J, DeForest CA
Nat Chem Biol. 2025 Oct 9. doi: 10.1038/s41589-025-02037-5. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
243769	mGreenLantern-A-SpyTag	Expresses multifunctional construct enabling the YES-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) treatment. Construct contains a SpyTag for material tethering.
243770	mGreenLantern-S-SpyTag	Expresses multifunctional construct enabling the YES-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) treatment. Construct contains a SpyTag for material tethering.
243771	mGreenLantern-T-SpyTag	Expresses multifunctional construct enabling the YES-gated release of mGreenLantern from materials in response to TEV treatment. Construct contains a SpyTag for material tethering.
243772	mGreenLantern-A OR S-SpyTag	Expresses multifunctional construct enabling the OR-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) OR sortase eSrtA(4S9) treatment. Contains a SpyTag for material tethering.
243773	mGreenLantern-A OR T-SpyTag	Expresses multifunctional construct enabling the OR-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) OR TEV treatment. Contains a SpyTag for material tethering.
243774	mGreenLantern-S OR T-SpyTag	Expresses multifunctional construct enabling the OR-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) OR TEV treatment. Contains a SpyTag for material tethering.
243775	mGreenLantern-A AND S-SpyTag	Expresses multifunctional construct enabling the AND-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND sortase eSrtA(4S9) treatment. Contains a SpyTag for material tethering.
243776	mGreenLantern-A AND T-SpyTag	Expresses multifunctional construct enabling the AND-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND TEV treatment. Contains a SpyTag for material tethering.
243777	mGreenLantern-S AND T-SpyTag	Expresses multifunctional construct enabling the AND-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) AND TEV treatment. Contains a SpyTag for material tethering.
243778	mGreenLantern- A AND (S OR T)-SpyTag	Expresses multifunctional construct enabling the AND(OR)-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND (sortase eSrtA(4S9) OR TEV) treatment. Contains a SpyTag for material tethering.
243779	mGreenLantern- S AND (A OR T)-SpyTag	Expresses multifunctional construct enabling the AND(OR)-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) AND (sortase eSrtA(2A9) OR TEV) treatment. Contains a SpyTag for material tethering.
243780	mGreenLantern- T AND (A OR S)-SpyTag	Expresses multifunctional construct enabling the AND(OR)-gated release of mGreenLantern from materials in response to TEV AND (sortase eSrtA(2A9) OR sortase eSrtA(4S9)) treatment. Contains a SpyTag for material tethering.
243781	mGreenLantern-A OR (S AND T)-SpyTag	Expresses multifunctional construct enabling the OR(AND)-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) OR (sortase eSrtA(4S9) AND TEV) treatment. Contains a SpyTag for material tethering.
243782	mGreenLantern-S OR (A AND T)-SpyTag	Expresses multifunctional construct enabling the OR(AND)-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) OR (sortase eSrtA(2A9) AND TEV) treatment. Contains a SpyTag for material tethering.
243783	mGreenLantern-T OR (A AND S)-SpyTag	Expresses multifunctional construct enabling the OR(AND)-gated release of mGreenLantern from materials in response to TEV OR (sortase eSrtA(2A9) AND sortase eSrtA(4S9) ) treatment. Contains a SpyTag for material tethering.
243784	mGreenLantern-A OR S OR T-SpyTag	Expresses multifunctional construct enabling the OR OR-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) OR sortase eSrtA(4S9) OR TEV treatment. Contains a SpyTag for material tethering.
243785	mGreenLantern-A AND S AND T-SpyTag	Expresses multifunctional construct enabling the AND AND-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND sortase eSrtA(4S9) AND TEV treatment. Contains a SpyTag for material tethering.
243786	mCherry-S-SpyTag	Expresses multifunctional construct enabling the YES-gated release of mCherry from materials in response to sortase eSrtA(4S9) treatment. Construct contains a SpyTag for material tethering.
243788	mCerulean-S AND T-SpyTag	Expresses multifunctional construct enabling the AND-gated release of mCerulean from materials in response to sortase eSrtA(4S9) AND TEV treatment. Contains a SpyTag for material tethering.
243789	mGreenLantern-[(A OR S) AND T] OR (C AND V)-SpyTag	Expresses multifunctional construct enabling the [AND/(OR)]OR(AND)-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9), sortase eSrtA(4S9), TEV, HRV-3C, TEV and TVMV proteases. Construct contains a SpyTag for material tethering.
243790	HER2Nb-C AND (A OR T)-SpyTag	Expresses multifunctional construct enabling the AND(OR)-gated release of a HER2 Nanobody from materials in response to HRV-3C AND (sortase eSrtA(2A9) OR TEV) treatment.
243791	mGreenLantern-C OR (T AND V)-CAAX	Expresses multifunctional construct enabling the OR(AND)-gated release of mGreenLantern from membranes in response to HRV-3C OR (TEV AND TVMV) treatment. Construct contains a CAAX tag for intracellular membrane tethering.
243792	HRV-3C Protease-mCherry	Encodes for mammalian expression of HRV-3C protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.
243793	TEV Protease-mCherry	Encodes for mammalian expression of TEV protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.
243794	TVMV Protease-mCherry	Encodes for mammalian expression of TVMV protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.
243795	TEV-HRV-3C Proteases-mCherry	Encodes for mammalian expression of TEV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.
243796	TVMV-HRV-3C Proteases-mCherry	Encodes for mammalian expression of TVMV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.
243797	TVMV-TEV Proteases-mCherry	Encodes for mammalian expression of TVMV and TEV protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.
243798	TVMV-TEV-HRV-3C Proteases-mCherry	Encodes for mammalian expression of TVMV, TEV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.