Boolean logic-gated protein presentation through autonomously compiled molecular topology.
Gharios R, Ross ML, Li A, Kottantharayil SP, Hoye J, DeForest CA
Nat Chem Biol. 2025 Oct 9. doi: 10.1038/s41589-025-02037-5.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 243769 | mGreenLantern-A-SpyTag | Expresses multifunctional construct enabling the YES-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) treatment. Construct contains a SpyTag for material tethering. |
| 243770 | mGreenLantern-S-SpyTag | Expresses multifunctional construct enabling the YES-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) treatment. Construct contains a SpyTag for material tethering. |
| 243771 | mGreenLantern-T-SpyTag | Expresses multifunctional construct enabling the YES-gated release of mGreenLantern from materials in response to TEV treatment. Construct contains a SpyTag for material tethering. |
| 243772 | mGreenLantern-A OR S-SpyTag | Expresses multifunctional construct enabling the OR-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) OR sortase eSrtA(4S9) treatment. Contains a SpyTag for material tethering. |
| 243773 | mGreenLantern-A OR T-SpyTag | Expresses multifunctional construct enabling the OR-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) OR TEV treatment. Contains a SpyTag for material tethering. |
| 243774 | mGreenLantern-S OR T-SpyTag | Expresses multifunctional construct enabling the OR-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) OR TEV treatment. Contains a SpyTag for material tethering. |
| 243775 | mGreenLantern-A AND S-SpyTag | Expresses multifunctional construct enabling the AND-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND sortase eSrtA(4S9) treatment. Contains a SpyTag for material tethering. |
| 243776 | mGreenLantern-A AND T-SpyTag | Expresses multifunctional construct enabling the AND-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND TEV treatment. Contains a SpyTag for material tethering. |
| 243777 | mGreenLantern-S AND T-SpyTag | Expresses multifunctional construct enabling the AND-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) AND TEV treatment. Contains a SpyTag for material tethering. |
| 243778 | mGreenLantern- A AND (S OR T)-SpyTag | Expresses multifunctional construct enabling the AND(OR)-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND (sortase eSrtA(4S9) OR TEV) treatment. Contains a SpyTag for material tethering. |
| 243779 | mGreenLantern- S AND (A OR T)-SpyTag | Expresses multifunctional construct enabling the AND(OR)-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) AND (sortase eSrtA(2A9) OR TEV) treatment. Contains a SpyTag for material tethering. |
| 243780 | mGreenLantern- T AND (A OR S)-SpyTag | Expresses multifunctional construct enabling the AND(OR)-gated release of mGreenLantern from materials in response to TEV AND (sortase eSrtA(2A9) OR sortase eSrtA(4S9)) treatment. Contains a SpyTag for material tethering. |
| 243781 | mGreenLantern-A OR (S AND T)-SpyTag | Expresses multifunctional construct enabling the OR(AND)-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) OR (sortase eSrtA(4S9) AND TEV) treatment. Contains a SpyTag for material tethering. |
| 243782 | mGreenLantern-S OR (A AND T)-SpyTag | Expresses multifunctional construct enabling the OR(AND)-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) OR (sortase eSrtA(2A9) AND TEV) treatment. Contains a SpyTag for material tethering. |
| 243783 | mGreenLantern-T OR (A AND S)-SpyTag | Expresses multifunctional construct enabling the OR(AND)-gated release of mGreenLantern from materials in response to TEV OR (sortase eSrtA(2A9) AND sortase eSrtA(4S9) ) treatment. Contains a SpyTag for material tethering. |
| 243784 | mGreenLantern-A OR S OR T-SpyTag | Expresses multifunctional construct enabling the OR OR-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) OR sortase eSrtA(4S9) OR TEV treatment. Contains a SpyTag for material tethering. |
| 243785 | mGreenLantern-A AND S AND T-SpyTag | Expresses multifunctional construct enabling the AND AND-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND sortase eSrtA(4S9) AND TEV treatment. Contains a SpyTag for material tethering. |
| 243786 | mCherry-S-SpyTag | Expresses multifunctional construct enabling the YES-gated release of mCherry from materials in response to sortase eSrtA(4S9) treatment. Construct contains a SpyTag for material tethering. |
| 243788 | mCerulean-S AND T-SpyTag | Expresses multifunctional construct enabling the AND-gated release of mCerulean from materials in response to sortase eSrtA(4S9) AND TEV treatment. Contains a SpyTag for material tethering. |
| 243789 | mGreenLantern-[(A OR S) AND T] OR (C AND V)-SpyTag | Expresses multifunctional construct enabling the [AND/(OR)]OR(AND)-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9), sortase eSrtA(4S9), TEV, HRV-3C, TEV and TVMV proteases. Construct contains a SpyTag for material tethering. |
| 243790 | HER2Nb-C AND (A OR T)-SpyTag | Expresses multifunctional construct enabling the AND(OR)-gated release of a HER2 Nanobody from materials in response to HRV-3C AND (sortase eSrtA(2A9) OR TEV) treatment. |
| 243791 | mGreenLantern-C OR (T AND V)-CAAX | Expresses multifunctional construct enabling the OR(AND)-gated release of mGreenLantern from membranes in response to HRV-3C OR (TEV AND TVMV) treatment. Construct contains a CAAX tag for intracellular membrane tethering. |
| 243792 | HRV-3C Protease-mCherry | Encodes for mammalian expression of HRV-3C protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence. |
| 243793 | TEV Protease-mCherry | Encodes for mammalian expression of TEV protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence. |
| 243794 | TVMV Protease-mCherry | Encodes for mammalian expression of TVMV protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence. |
| 243795 | TEV-HRV-3C Proteases-mCherry | Encodes for mammalian expression of TEV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence. |
| 243797 | TVMV-TEV Proteases-mCherry | Encodes for mammalian expression of TVMV and TEV protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence. |
| 243798 | TVMV-TEV-HRV-3C Proteases-mCherry | Encodes for mammalian expression of TVMV, TEV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence. |