Sai Reddy Lab: A yeast mating-based platform enables the generation and screening of ultra large antibody libraries
Lester Frei, Sai Tosa Reddy
bioRxiv
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 243802 | pI-SceI | Contains the ORF for the homing endonuclease I-SceI under the control of the strong, constitutive yeast promoter PTDH3. |
| 243803 | pLFYa | Dropout vector for genomic integration into yeast strain LFYa. GFP is flanked by BamHI sites for easy cloning using Gibson assembly. Construct contains the attP site for the serine recombinase BxB1. |
| 243804 | pLFYalpha | Dropout vector for genomic integration into yeast strain LFYalpha. GFP is flanked by BamHI sites for easy cloning using Gibson assembly. Construct contains attB site for the serine recombinase BxB1. |