GoldenBraid2.0 E. coli: a comprehensive and characterized toolkit for enterics.
Cooke MB, Welch KT, Ramirez LD, Wen AX, Marciano DC, Herman C
Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf015. doi: 10.1093/synbio/ysaf015. eCollection 2025.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 243832 | pR6Ka1_Red | GoldenBraid2.0 Cloning vector. |
| 243833 | pR6Ka2_Red | GoldenBraid2.0 Cloning vector. |
| 243834 | pBSKa1_FRTampFRT | GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit. |
| 243836 | pBSKa1_FRTcamFRT | GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit. |
| 243837 | pBSKa1_FRTtetFRT | GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit. |
| 243838 | pBSKa1_FRTspcFRT | GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit. |
| 243839 | pBSKa2_FRTampFRT | GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit. |
| 243840 | pBSKa2_FRTkanFRT | GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit. |
| 243841 | pBSKa2_FRTcamFRT | GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit. |
| 243843 | pBSKa2_FRTspcFRT | GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit. |
| 243844 | pBSKa1_Stuffer1 | GoldenBraid2.0 Full transcription unit stuffer in an alpha-level cloning vector. |
| 243847 | pBSKa2_Stuffer2 | GoldenBraid2.0 Full transcription unit stuffer in an alpha-level cloning vector. |
| 243849 | p15Aa2_Blue | GoldenBraid2.0 expression vector. |
| 243850 | p15Ao1_Blue | GoldenBraid2.0 expression vector. |
| 243851 | p15Ao2_Blue | GoldenBraid2.0 expression vector. |
| 243852 | p15Aa1_Red | GoldenBraid2.0 expression vector. |
| 243853 | p15Aa2_Red | GoldenBraid2.0 expression vector. |
| 243854 | p15Ao1_Red | GoldenBraid2.0 expression vector. |
| 243855 | p15Ao2_Red | GoldenBraid2.0 expression vector. |
| 243856 | pMB1a1_Blue | GoldenBraid2.0 expression vector. |
| 243857 | pMB1a2_Blue | GoldenBraid2.0 expression vector. |
| 243858 | pMB1o1_Blue | GoldenBraid2.0 expression vector. |
| 243859 | pMB1o2_Blue | GoldenBraid2.0 expression vector. |
| 243864 | pSC101TSa1_Blue | GoldenBraid2.0 expression vector. |
| 243865 | pSC101TSa2_Blue | GoldenBraid2.0 expression vector. |
| 243866 | pSC101TSo1_Blue | GoldenBraid2.0 expression vector. |
| 243867 | pSC101TSo2_Blue | GoldenBraid2.0 expression vector. |
| 243868 | pSC101TSa1_Red | GoldenBraid2.0 expression vector. |
| 243869 | pSC101TSa2_Red | GoldenBraid2.0 expression vector. |
| 243870 | pSC101TSo1_Red | GoldenBraid2.0 expression vector. |
| 243872 | ptetgRNA-FRT | sgRNA expression vector under tetR transcriptional control. |
| 243875 | pBSKo2_Red | GoldenBraid2.0 Cloning vector. |
| 243876 | pR6Ko1_Red | GoldenBraid2.0 Cloning vector. |
| 243877 | pR6Ko2_Red | GoldenBraid2.0 Cloning vector. |
| 243878 | pUPD3_FULLUNIT_Stuffer1 | GoldenBraid2.0 Transcriptionally-inert stuffer part. |
| 243879 | pUPD3_FULLUNIT_Stuffer2 | GoldenBraid2.0 Transcriptionally-inert stuffer part. |
| 243880 | pUPD3_FULLPRM_Stuffer3 | GoldenBraid2.0 Transcriptionally-inert stuffer part. |
| 243881 | pUPD3_FULLPRM_Stuffer4 | GoldenBraid2.0 Transcriptionally-inert stuffer part. |
| 243882 | pUPD3_PARTPRM_Stuffer5 | GoldenBraid2.0 Transcriptionally-inert stuffer part. |
| 243884 | pUPD3_RBS_Stuffer7 | GoldenBraid2.0 Transcriptionally-inert stuffer part. |
| 243885 | pUPD3_RBS_Stuffer8 | GoldenBraid2.0 Transcriptionally-inert stuffer part. |
| 243886 | pUPD3_CDS_Stuffer9 | GoldenBraid2.0 Transcriptionally-inert stuffer part. |
| 243887 | pUPD3_CDS_Stuffer10 | GoldenBraid2.0 Transcriptionally-inert stuffer part. |
| 243888 | pUPD3_FULLTRM_Stuffer11 | GoldenBraid2.0 Transcriptionally-inert stuffer part. |
| 243889 | pUPD3_FULLTRM_Stuffer12 | GoldenBraid2.0 Transcriptionally-inert stuffer part. |
| 243890 | pUPD3_RTAGTRM_Stuffer13 | GoldenBraid2.0 Transcriptionally-inert stuffer part. |
| 243891 | pUPD3_RTAGTRM_Stuffer14 | GoldenBraid2.0 Transcriptionally-inert stuffer part. |
| 243892 | pUPD3_DTAGTRM_Stuffer15 | GoldenBraid2.0 Transcriptionally-inert stuffer part. |
| 243893 | pUPD3_DTAGTRM_Stuffer16 | GoldenBraid2.0 Transcriptionally-inert stuffer part. |
| 243894 | pUPD3_RDTAGTRM_Stuffer17 | GoldenBraid2.0 Transcriptionally-inert stuffer part. |
| 243895 | pUPD3_RDTAGTRM_Stuffer18 | GoldenBraid2.0 Transcriptionally-inert stuffer part. |
| 243896 | pBSKo1_Stuffer1_Stuffer2 | GoldenBraid2.0 Full transcription unit stuffer in an omega-level cloning vector. |
| 243897 | pBSKo2_Stuffer2_Stuffer1 | GoldenBraid2.0 Full transcription unit stuffer in an omega-level cloning vector. |
| 243898 | pR6Ko1_FRTampFRT | GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts. |
| 243899 | pR6Ko1_FRTkanFRT | GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts. |
| 243902 | pR6Ko1_FRTspcFRT | GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts. |
| 243908 | pUPD3_FULLPRM_Pem7 | GoldenBraid2.0 Strong, constitutive promoter, including ribosome binding site. |
| 243909 | pUPD3_FULLPRM_lacI_Plac | GoldenBraid2.0 IPTG/Lactose-Inducible promoter, including ribosome binding site. |
| 243910 | pUPD3_FULLPRM_Plac | GoldenBraid2.0 IPTG/Lactose-Inducible promoter, including ribosome binding site. Lacks repressor, LacI. |
| 243911 | pUPD3_FULLPRM_araC_Para | GoldenBraid2.0 Arabinose-Inducible promoter, including ribosome binding site. |
| 243912 | pUPD3_FULLPRM_Para | GoldenBraid2.0 Arabinose-Inducible promoter, including ribosome binding site. Lacks activator protein, AraC. |
| 243913 | pUPD3_FULLPRM_tetR_Ptet | GoldenBraid2.0 Tetracycline/Anhydrotetracycline-Inducible promoter, including ribosome binding site. |
| 243914 | pUPD3_FULLPRM_Ptet | GoldenBraid2.0 Tetracycline/Anhydrotetracycline-Inducible promoter, including ribosome binding site. Lacks repressor, TetR. |
| 243915 | pUPD3_FULLPRM_cI857_PR | GoldenBraid2.0 Temperature-Inducible promoter, including ribosome binding site. |
| 243916 | pUPD3_FULLPRM_PR | GoldenBraid2.0 Temperature-Inducible promoter, including ribosome binding site. Lacks repressor, cI857. |
| 243917 | pUPD3_FULLPRM_PT7 | GoldenBraid2.0 T7 RNA polymerase-dependent promoter, including ribosome binding site. |
| 243918 | pUPD3_FULLPRM_PT7lac | GoldenBraid2.0 T7 RNA polymerase and IPTG/Lactose-dependent promoter, including ribosome binding site. |
| 243919 | pUPD3_PARTPRM_Pem7 | GoldenBraid2.0 Strong, constitutive promoter, not including ribosome binding site. |
| 243920 | pUPD3_PARTPRM_lacI_Plac | GoldenBraid2.0 IPTG/Lactose-Inducible promoter, not including ribosome binding site. |
| 243921 | pUPD3_PARTPRM_Plac | GoldenBraid2.0 IPTG/Lactose-Inducible promoter, not including ribosome binding site. Lacks repressor, LacI. |
| 243923 | pUPD3_PARTPRM_Para | GoldenBraid2.0 Arabinose-Inducible promoter, not including ribosome binding site. Lacks activator protein, AraC. |
| 243924 | pUPD3_PARTPRM_tetR_Ptet | GoldenBraid2.0 Tetracycline/Anhydrotetracycline-Inducible promoter, not including ribosome binding site. |
| 243926 | pUPD3_PARTPRM_cI857_PR | GoldenBraid2.0 Temperature-Inducible promoter, not including ribosome binding site. |
| 243927 | pUPD3_PARTPRM_PR | GoldenBraid2.0 Temperature-Inducible promoter, not including ribosome binding site. Lacks repressor, cI857. |
| 243928 | pUPD3_PARTPRM_PT7 | GoldenBraid2.0 T7 RNA polymerase-dependent promoter, not including ribosome binding site. |
| 243929 | pUPD3_PARTPRM_PT7lac | GoldenBraid2.0 T7 RNA polymerase and IPTG/Lactose-dependent promoter, not including ribosome binding site. |
| 243930 | pUPD3_PARTPRM_J23100 | GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site. |
| 243931 | pUPD3_PARTPRM_J23101 | GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site. |
| 243932 | pUPD3_PARTPRM_J23102 | GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site. |
| 243933 | pUPD3_PARTPRM_J23103 | GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site. |
| 243934 | pUPD3_PARTPRM_J23104 | GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site. |
| 243935 | pUPD3_PARTPRM_J23105 | GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site. |
| 243936 | pUPD3_PARTPRM_J23106 | GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site. |
| 243937 | pUPD3_PARTPRM_J23107 | GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site. |
| 243938 | pUPD3_PARTPRM_J23108 | GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site. |
| 243939 | pUPD3_PARTPRM_J23109 | GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site. |
| 243940 | pUPD3_PARTPRM_J23110 | GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site. |
| 243941 | pUPD3_PARTPRM_J23111 | GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site. |
| 243942 | pUPD3_PARTPRM_J23113 | GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site. |
| 243943 | pUPD3_PARTPRM_J23114 | GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site. |
| 243944 | pUPD3_PARTPRM_J23115 | GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site. |
| 243945 | pUPD3_PARTPRM_J23116 | GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site. |
| 243946 | pUPD3_PARTPRM_J23117 | GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site. |
| 243947 | pUPD3_PARTPRM_J23118 | GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site. |
| 243948 | pUPD3_PARTPRM_J23119 | GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site. |
| 243949 | pUPD3_RBS_J61100 | GoldenBraid2.0 Ribosome binding site. |
| 243950 | pUPD3_RBS_J61101 | GoldenBraid2.0 Ribosome binding site. |
| 243951 | pUPD3_RBS_J61102 | GoldenBraid2.0 Ribosome binding site. |
| 243952 | pUPD3_RBS_J61103 | GoldenBraid2.0 Ribosome binding site. |
| 243953 | pUPD3_RBS_J61104 | GoldenBraid2.0 Ribosome binding site. |
| 243954 | pUPD3_RBS_J61105 | GoldenBraid2.0 Ribosome binding site. |
| 243955 | pUPD3_RBS_J61106 | GoldenBraid2.0 Ribosome binding site. |
| 243956 | pUPD3_RBS_J61107 | GoldenBraid2.0 Ribosome binding site. |
| 243957 | pUPD3_RBS_J61108 | GoldenBraid2.0 Ribosome binding site. |
| 243958 | pUPD3_RBS_J61109 | GoldenBraid2.0 Ribosome binding site. |
| 243959 | pUPD3_RBS_J61110 | GoldenBraid2.0 Ribosome binding site. |
| 243960 | pUPD3_RBS_J61111 | GoldenBraid2.0 Ribosome binding site. |
| 243961 | pUPD3_RBS_J61112 | GoldenBraid2.0 Ribosome binding site. |
| 243962 | pUPD3_RBS_J61113 | GoldenBraid2.0 Ribosome binding site. |
| 243963 | pUPD3_RBS_J61114 | GoldenBraid2.0 Ribosome binding site. |
| 243964 | pUPD3_RBS_J61115 | GoldenBraid2.0 Ribosome binding site. |
| 243965 | pUPD3_RBS_J61116 | GoldenBraid2.0 Ribosome binding site. |
| 243966 | pUPD3_RBS_J61117 | GoldenBraid2.0 Ribosome binding site. |
| 243967 | pUPD3_RBS_J61118 | GoldenBraid2.0 Ribosome binding site. |
| 243968 | pUPD3_RBS_J61119 | GoldenBraid2.0 Ribosome binding site. |
| 243969 | pUPD3_RBS_J61120 | GoldenBraid2.0 Ribosome binding site. |
| 243970 | pUPD3_RBS_J61121 | GoldenBraid2.0 Ribosome binding site. |
| 243971 | pUPD3_RBS_J61122 | GoldenBraid2.0 Ribosome binding site. |
| 243972 | pUPD3_RBS_J61123 | GoldenBraid2.0 Ribosome binding site. |
| 243973 | pUPD3_RBS_J61124 | GoldenBraid2.0 Ribosome binding site. |
| 243974 | pUPD3_RBS_J61125 | GoldenBraid2.0 Ribosome binding site. |
| 243975 | pUPD3_RBS_J61126 | GoldenBraid2.0 Ribosome binding site. |
| 243976 | pUPD3_RBS_J61127 | GoldenBraid2.0 Ribosome binding site. |
| 243977 | pUPD3_RBS_J61128 | GoldenBraid2.0 Ribosome binding site. |
| 243978 | pUPD3_RBS_J61129 | GoldenBraid2.0 Ribosome binding site. |
| 243979 | pUPD3_RBS_J61130 | GoldenBraid2.0 Ribosome binding site. |
| 243980 | pUPD3_RBS_J61131 | GoldenBraid2.0 Ribosome binding site. |
| 243981 | pUPD3_RBS_J61132 | GoldenBraid2.0 Ribosome binding site. |
| 243982 | pUPD3_RBS_J61133 | GoldenBraid2.0 Ribosome binding site. |
| 243983 | pUPD3_RBS_J61134 | GoldenBraid2.0 Ribosome binding site. |
| 243984 | pUPD3_RBS_J61135 | GoldenBraid2.0 Ribosome binding site. |
| 243985 | pUPD3_RBS_J61136 | GoldenBraid2.0 Ribosome binding site. |
| 243986 | pUPD3_RBS_J61137 | GoldenBraid2.0 Ribosome binding site. |
| 243987 | pUPD3_RBS_J61138 | GoldenBraid2.0 Ribosome binding site. |
| 243988 | pUPD3_RBS_J61139 | GoldenBraid2.0 Ribosome binding site. |
| 243989 | pUPD3_RBS_RBS1 | GoldenBraid2.0 Ribosome binding site. |
| 243990 | pUPD3_RBS_RBS2 | GoldenBraid2.0 Ribosome binding site. |
| 243991 | pUPD3_RBS_RBS3 | GoldenBraid2.0 Ribosome binding site. |
| 243992 | pUPD3_RBS_RBS4 | GoldenBraid2.0 Ribosome binding site. |
| 243993 | pUPD3_RBS_RBS5 | GoldenBraid2.0 Ribosome binding site. |
| 243994 | pUPD3_RBS_RBS6 | GoldenBraid2.0 Ribosome binding site. |
| 243995 | pUPD3_RBS_RBS7 | GoldenBraid2.0 Ribosome binding site. |
| 243996 | pUPD3_RBS_RBS8 | GoldenBraid2.0 Ribosome binding site. |
| 243997 | pUPD3_RBS_RBS9 | GoldenBraid2.0 Ribosome binding site. |
| 243998 | pUPD3_RBS_RBS10 | GoldenBraid2.0 Ribosome binding site. |
| 243999 | pUPD3_RBS_RBS11 | GoldenBraid2.0 Ribosome binding site. |
| 244000 | pUPD3_RBS_RBS12 | GoldenBraid2.0 Ribosome binding site. |
| 244001 | pUPD3_RBS_RBS13 | GoldenBraid2.0 Ribosome binding site. |
| 244002 | pUPD3_RBS_RBS14 | GoldenBraid2.0 Ribosome binding site. |
| 244003 | pUPD3_RBS_RBS15 | GoldenBraid2.0 Ribosome binding site. |
| 244004 | pUPD3_RBS_RBS16 | GoldenBraid2.0 Ribosome binding site. |
| 244005 | pUPD3_RBS_RBS17 | GoldenBraid2.0 Ribosome binding site. |
| 244006 | pUPD3_RBS_RBS18 | GoldenBraid2.0 Ribosome binding site. |
| 244007 | pUPD3_RBS_RBS19 | GoldenBraid2.0 Ribosome binding site. |
| 244008 | pUPD3_RBS_RBS20 | GoldenBraid2.0 Ribosome binding site. |
| 244009 | pUPD3_RBS_RBS21 | GoldenBraid2.0 Ribosome binding site. |
| 244010 | pUPD3_RBS_RBS22 | GoldenBraid2.0 Ribosome binding site. |
| 244011 | pUPD3_RBS_RBS23 | GoldenBraid2.0 Ribosome binding site. |
| 244012 | pUPD3_RBS_RBS24 | GoldenBraid2.0 Ribosome binding site. |
| 244013 | pUPD3_RBS_RBS25 | GoldenBraid2.0 Ribosome binding site. |
| 244014 | pUPD3_RBS_RBS26 | GoldenBraid2.0 Ribosome binding site. |
| 244015 | pUPD3_RBS_RBS27 | GoldenBraid2.0 Ribosome binding site. |
| 244016 | pUPD3_RBS_RBS28 | GoldenBraid2.0 Ribosome binding site. |
| 244017 | pUPD3_CDS_lacI | GoldenBraid2.0 IPTG/Lactose-dependent transcriptional repressor. |
| 244018 | pUPD3_CDS_araC | GoldenBraid2.0 Arabinose-dependent transcriptional activator. |
| 244019 | pUPD3_CDS_tetR | GoldenBraid2.0 Tetracycline/Anhydrotetracycline-dependent transcriptional repressor. |
| 244020 | pUPD3_CDS_cI857 | GoldenBraid2.0 Temperature-dependent transcriptional repressor. |