Increasing Cas12a- and Cas9-mediated deletion sizes with exonucleases.
Wang X, Clem J, Malzahn A, Qi Y
Plant Physiol. 2025 Oct 31;199(3):kiaf507. doi: 10.1093/plphys/kiaf507.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 244022 | pYPQ166-TREX2-N | Gateway entry plasmid (attL1& attR5) expressing TREX2 exonuclease fused to the N-terminus of zSpCas9, connected by a flexible XTEN linker, without promoter |
| 244023 | pYPQ166-AtEXO1B-N | Gateway entry plasmid (attL1& attR5) expressing AtEXO1B exonuclease fused to the N-terminus of zSpCas9, connected by a flexible XTEN linker, without promoter |
| 244024 | pYPQ230-AtEXO1B-N | Gateway entry plasmid (attL1& attR5) expressing AtEXO1B exonuclease fused to the N-terminus of LbCas12a, connected by a flexible XTEN linker, without promoter |
| 244025 | pYPQ230-TREX2-N | Gateway entry plasmid (attL1& attR5) expressing TREX2 exonuclease fused to the N-terminus of LbCas12a, connected by a flexible XTEN linker, without promoter |