Ionizable networks mediate pH-dependent allostery in the SH2 domain-containing signaling proteins SHP2 and SRC.
Van Dyck PK, Piszkin L, Gorski EA, Nascimento ET, Abebe JA, Hoffmann LM, Peng JW, White KA
Sci Signal. 2025 Nov 11;18(912):eadt3018. doi: 10.1126/scisignal.adt3018. Epub 2025 Nov 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 244491 | pGEX-4T1 SHP2 E252A | Bacterial Expression of SHP2 mutant |
| 244492 | pGEX-4T1 GST-SHP2 H116A | Bacterial Expression of SHP2 mutant |
| 244493 | pGEX-4T1 GST-SHP2 H116A E252A | Bacterial Expression of pH insensitive SHP2 mutant |
| 246423 | Flag-Src C185A | Expresses Src mutant C185A or C188A in Mammalian cells |
| 246424 | Flag-Src H201A | Expresses Src mutant H201A (aka H204A) in Mammalian cells |
| 246425 | Flag- Src H492A | Expresses Src mutant H492A (aka H495A) in Mammalian cells |
| 246426 | Flag-Src E524A | Expresses Src mutant E524A (aka E527A) in Mammalian cells |
| 246427 | Flag-Src C185A H201A H492A E524A | Expresses Src pH insensitive mutant in Mammalian cells |
| 246428 | Flag-Src Y527E | Expresses Src mutant Y527E (aka Y530E) in Mammalian cells |
| 246429 | Flag-Src Y527A | Expresses Src mutant Y527A (aka Y530A) in Mammalian cells |