Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing.
Shi H, Al-Sayyad N, Wasko KM, Trinidad MI, Doherty EE, Vohra K, Boger RS, Colognori D, Cofsky JC, Skopintsev P, Bryant Z, Doudna JA
Mol Cell. 2025 May 1;85(9):1730-1742.e9. doi: 10.1016/j.molcel.2025.03.024. Epub 2025 Apr 23.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 244825 | pHS1_10xHis_MBP_TEV_WT-Cas9 | Bacterial expression of SpyCas9 (WT) for protein purification |
| 244826 | pHS23_10xHis_MBP_TEV_SpG-Cas9 | Bacterial expression of SpG Cas9 for protein purification |
| 244827 | pHS24_10xHis_MBP_TEV_SpRY-Cas9 | Bacterial expression of SpRY Cas9 for protein purification |
| 244828 | pHS25_10xHis_MBP_TEV_EQR-Cas9 | Bacterial expression of EQR Cas9 for protein purification |
| 244829 | pHS26_10xHis_MBP_TEV_VRER-Cas9 | Bacterial expression of VRER Cas9 for protein purification |
| 244830 | pHS16_10xHis_MBP_TEV_xPBA-Cas9 | Bacterial expression of xPBA Cas9 for protein purification |
| 244831 | pHS9_10xHis_MBP_TEV_dCas9 | Bacterial expression of catalytically inactive Cas9 (dCas9) for protein purification |
| 244832 | pHS484_10xHis_MBP_TEV_dSpRY | Bacterial expression of catalytically inactive SpRY Cas9 (dSpRY) for protein purification |
| 244833 | pHS71_10xHis_MBP_TEV_WT-Cas9_2xNLS | Bacterial expression of SpyCas9 (WT) with 2xSV40 nuclear localization sequence for protein purification |
| 244834 | pHS72_10xHis_MBP_TEV_SpG-Cas9_2xNLS | Bacterial expression of SpG Cas9 with 2xSV40 nuclear localization sequence for protein purification |
| 244835 | pHS73_10xHis_MBP_TEV_SpRY-Cas9_2xNLS | Bacterial expression of SpRY Cas9 with 2xSV40 nuclear localization sequence for protein purification |