Directed evolution expands CRISPR-Cas12a genome-editing capacity.
Ma E, Chen K, Shi H, Wasko KM, Esain-Garcia I, Trinidad MI, Zhou K, Ye J, Doudna JA
Nucleic Acids Res. 2025 Jul 8;53(13):gkaf649. doi: 10.1093/nar/gkaf649.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 244836 | pEM1_Euk_2NLS-WT ( LbCas12a)-2NLS | Mammalian expression of wild-type LbCas12a with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus |
| 244837 | pEM2_Euk_2NLS-RVQ (LbCas12a)-2NLS | Mammalian expression of LbCas12a-RVQ with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus |
| 244838 | pEM3_Euk_2NLS-Flex-Cas12a (LbCas12a)-2NLS | Mammalian expression of Flex-Cas12a with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus |
| 244839 | pEM4_CL7_Bac_2NLS-WT (LbCas12a)-4NLS | Bacterial expression of wild-type LbCas12a with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus |
| 244840 | pEM5_CL7_Bac_2NLS-Lbm6 (LbCas12a)-4NLS | Bacterial expression of Lbm6 LbCas12a with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus |
| 244841 | pEM6_CL7_Bac_2NLS-RVQ (LbCas12a)-2NLS | Bacterial expression of LbCas12a-RVQ with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus |
| 244843 | pEM8_2CTH10_Bac_Lbm6 (LbCas12a)-4NLS | Bacterial expression of Lbm6-LbCas12a with 10xHis-MBP at N-temrinus and 4xSV40 NLS at C-temrinus |
| 244844 | pEM9_CL7_Bac_2NLS-iCas12a (LbCas12a)-4NLS | Bacterial expression of iCas12a LbCas12a with 2xSV40 nuclear localization sequence for protein purification |
| 244845 | pHS638_Flex-Cas12a-CBE | Mammalian expression of Flex-Cas12a cytosine base editor |
| 244846 | pHS641_Flex-Cas12a-ABE | Mammalian expression of Flex-Cas12a adenine base editor |