A live-cell biosensor of in vivo receptor tyrosine kinase activity reveals feedback regulation of a developmental gradient.
Ho EK, Kim-Yip RP, Simpkins AG, Farahani PE, Oatman HR, Posfai E, Shvartsman SY, Toettcher JE
Cell Rep. 2025 Jul 22;44(7):115930. doi: 10.1016/j.celrep.2025.115930. Epub 2025 Jun 27.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
244932 | pU6-BbsI-EGFR | CRISPR gRNA targeting the C-terminal end of EGFR for cleavage. |
244933 | pU6-BbsI-Torso | CRISPR gRNA targeting the C-terminal end of Torso for cleavage. |
244934 | pU6-BbsI-Btl | CRISPR gRNA targeting the C-terminal end of Breathless for cleavage. |