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A live-cell biosensor of in vivo receptor tyrosine kinase activity reveals feedback regulation of a developmental gradient.

Ho EK, Kim-Yip RP, Simpkins AG, Farahani PE, Oatman HR, Posfai E, Shvartsman SY, Toettcher JE
Cell Rep. 2025 Jul 22;44(7):115930. doi: 10.1016/j.celrep.2025.115930. Epub 2025 Jun 27. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
244932pU6-BbsI-EGFRCRISPR gRNA targeting the C-terminal end of EGFR for cleavage.
244933pU6-BbsI-TorsoCRISPR gRNA targeting the C-terminal end of Torso for cleavage.
244934pU6-BbsI-BtlCRISPR gRNA targeting the C-terminal end of Breathless for cleavage.

Antibodies from Article