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A three dimensional immunolabeling method with peroxidase-fused nanobodies and fluorochromized tyramide-glucose oxidase signal amplification.

Yamauchi K, Koike M, Hioki H
Commun Biol. 2025 Jun 18;8(1):903. doi: 10.1038/s42003-025-08317-z. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
244973pCAG-GFP1 POD-nAb-WPREExpresses a fusion protein of a nanobody against GFP and peroxidase (POD) in mammalian cells
244974pCAG-RFP6 POD-nAb-WPREExpresses a fusion protein of a nanobody against RFP and peroxidase (POD) in mammalian cells
244975pCAG-ITGAM POD-nAb-WPREExpresses a fusion protein of a nanobody against integrin alpha M (ITGAM; CD11b) and peroxidase (POD) in mammalian cells

Antibodies from Article