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JIP4 and RILPL1 utilize opposing motor force to dynamically regulate lysosomal tubulation.

Bonet-Ponce L, Tegicho T, Fernandez-Martinez N, Rozenberg IA, Ashriem M, Beilina A, Kluss JH, Li Y, Cookson MR
J Cell Biol. 2025 Nov 3;224(11):e202404018. doi: 10.1083/jcb.202404018. Epub 2025 Sep 24. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
244978mBaoJin-JIP4Express JIP4 in mammalian cells with mBaoJin tag (monomeric StayGold)
244980mCherry-RILPL1Express RILPL1 in mammalian cells with mCherry tag
244981mCherry-RILPL1 (R293A)Express RILPL1 in mammalian cells with mCherry tag expressing a R293A mutation
2449822xmyc-RILPL1Express RILPL1 in mammalian cells with 2xmyc tag
2449842xmyc-RAB10 (T73A)Express RAB10 in mammalian cells with 2xmyc tag and T73A mutation
244985mBaoJin-RILPL1Express RILPL1 in mammalian cells with mBaoJin tag (monomeric StayGold)

Antibodies from Article