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The locus coeruleus maintains core body temperature and protects against hypothermia during dexmedetomidine-induced sedation.

Anuncibay Soto B, Ma Y, Nollet M, Wong S, Miracca G, Rastinejad D, Yustos R, Vyssotski AL, Franks NP, Wisden W
Proc Natl Acad Sci U S A. 2025 Oct 14;122(41):e2422878122. doi: 10.1073/pnas.2422878122. Epub 2025 Oct 7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
245063pAAV-hsynapsin-DIO-adra2a-shRNA-mCherryAAV-transgene knocking down adra2a receptor (adrenergic 2a receptor) transcripts cell type-selectively. Using the pPRIME. system, this generates mir30-derived shRNAs and a marker from the same RNA.
245064pAAV-hsynapsin-DIO-scramble shRNA - mCherryAAV transgene designes to express scramble (control) shRNA sequence cell-type selectively. Scramble hairpin expressed from the mir30 cassette together with mCherry.

Antibodies from Article