DNA damage drives antigen diversification in Trypanosoma brucei
Smith JE, Wang KJ, Kennedy EM, Munday JC, Singer L, Hakim JMC, So J, Beaver AK, Magesh A, Gilligan-Steinberg SD, Zheng J, Zhang B, Moorthy DN, Brown ZE, Akin EH, Mwakibete L, McCulloch R, Mugnier MR
Nature (2026)
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 245102 | pLEW100v5-BSD-FLAG-La-Cas9 | Inserts tetracycline-inducible codon optimized, nuclceus targeted FLAG-Cas9 into T. brucei cells at the rDNA spacer |
| 245103 | pUC19-HYG-BES1-AnTat1.1-telo | Inserts VSG AnTat1.1 in T. brucei cells at Lister427 BES1 |
| 245104 | pUC19-HYG-BES1-VSG-228-telo | Inserts VSG-228 in T. brucei cells at Lister427 BES1 |
| 245105 | pHD309-PUR-VSG-228 | Inserts VSG-228 coding sequence in T. brucei tubulin array |
| 245106 | pLEW100v5-PUR-VSG-228 | Inserts VSG-228 coding sequence in T. brucei rDNA spacer |
| 245107 | pLEW100v5-177-PUR-VSG-228 | Inserts VSG-228 coding sequence in T. brucei minichromosomes using the 177bp repeats |
| 245108 | pLEW100v5-PUR-T7-sgRNA | Plasmid backbone to for inserting a T7 promoter driven guide RNA into the rDNA spacer of T. brucei |
| 245109 | pLEW100v5-PUR-VSG-228.noUTRS | Inserts VSG-228 coding sequence without UTRs in T. brucei rDNA spacer |
| 245110 | pLEW100v5-PUR-VSG-228.500bp | Inserts 500 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694 |
| 245111 | pLEW100v5-PUR-VSG-228.400bp | Inserts 400 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694 |
| 245112 | pLEW100v5-PUR-VSG-228.300bp | Inserts 300 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694 |
| 245113 | pLEW100v5-PUR-VSG-228.200bp | Inserts 200 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694 |
| 245114 | pLEW100v5-PUR-VSG-228.100bp | Inserts 100 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694 |
| 245115 | pLEW100v5-PUR-VSG-228.100bp_offset | Inserts 100 bp of VSG-228 coding sequence in T. brucei rDNA spacer overlapping AnTat1.1 position 694, centered around common VSG-228 insertion |
| 245116 | pLEW100v5-BSD-VSG-228 | Inserts VSG-228 coding sequence in T. brucei rDNA spacer |
| 245117 | pLEW100v5-NEO-VSG-228 | Inserts VSG-228 coding sequence in T. brucei rDNA spacer |
| 245118 | pLEW100v5-NEO-T7-sgRNA | Plasmid backbone to for inserting a T7 promoter driven guide RNA into the rDNA spacer of T. brucei |
| 245119 | pLEW100v5-BSD-T7-sgRNA | Plasmid backbone to for inserting a T7 promoter driven guide RNA into the rDNA spacer of T. brucei |