The pathogenic T42A mutation in SHP2 rewires the interaction specificity of its N-terminal regulatory domain.
van Vlimmeren AE, Voleti R, Chartier CA, Jiang Z, Karandur D, Humphries PA, Lo WL, Shah NH
Proc Natl Acad Sci U S A. 2024 Jul 23;121(30):e2407159121. doi: 10.1073/pnas.2407159121. Epub 2024 Jul 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 245182 | pET-SUMO-SHP2-N-SH2(WT)-Avi | Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain |
| 245183 | pET-SUMO-SHP2-N-SH2(T42A)-Avi | Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain |
| 245184 | pET-SUMO-SHP2-N-SH2(L43F)-Avi | Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain |
| 245185 | pET-SUMO-SHP2-N-SH2(T52S)-Avi | Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain |
| 245186 | pET-SUMO-SHP2-N-SH2(K55R)-Avi | Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain |
| 245187 | pET-SUMO-SHP2-N-SH2(E76K)-Avi | Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain |
| 245188 | pET-SUMO-SHP2-C-SH2(WT)-Avi | Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain |
| 245189 | pET-SUMO-SHP2-C-SH2(R138Q)-Avi | Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain |
| 245190 | pET-SUMO-SHP2-C-SH2(E139D)-Avi | Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain |
| 245191 | pET-28b His6-TEV-SHP2-FL(WT) | Production of His-tagged full-length SHP2 |
| 245195 | pET-28b His6-TEV-SHP2-FL(T42A) | Production of His-tagged full-length SHP2 |
| 245196 | pET-28b His6-TEV-SHP2-FL(L43F) | Production of His-tagged full-length SHP2 |
| 245197 | pET-28b His6-TEV-SHP2-FL(T52S) | Production of His-tagged full-length SHP2 |
| 245201 | pET-28b His6-TEV-SHP2-FL(E76K) | Production of His-tagged full-length SHP2 |
| 245206 | pET-28b His6-TEV-SHP2-FL(R138Q) | Production of His-tagged full-length SHP2 |
| 245208 | pET-28b His6-TEV-SHP2-FL(E139D) | Production of His-tagged full-length SHP2 |
| 245220 | pEF myc-SHP2(WT) | Mammalian expression of myc-tagged full-length SHP2 |
| 245223 | pEF myc-SHP2(T42A) | Mammalian expression of myc-tagged full-length SHP2 |
| 245227 | pEF myc-SHP2(E76K) | Mammalian expression of myc-tagged full-length SHP2 |
| 245239 | pMSCV-SHP2(T42A) full-length-PIG | Production of viral vector encoding full-length SHP2 |
| 245240 | pMSCV-SHP2(E76K) full-length-PIG | Production of viral vector encoding full-length SHP2 |