Deep mutational scanning of the multi-domain phosphatase SHP2 reveals mechanisms of regulation and pathogenicity.
Jiang Z, van Vlimmeren AE, Karandur D, Semmelman A, Shah NH
Nat Commun. 2025 Jul 1;16(1):5464. doi: 10.1038/s41467-025-60641-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 245198 | pET-28b His6-TEV-SHP2-FL(D61Y) | Production of His-tagged full-length SHP2 |
| 245199 | pET-28b His6-TEV-SHP2-FL(E69K) | Production of His-tagged full-length SHP2 |
| 245200 | pET-28b His6-TEV-SHP2-FL(A72V) | Production of His-tagged full-length SHP2 |
| 245202 | pET-28b His6-TEV-SHP2-FL(C104P) | Production of His-tagged full-length SHP2 |
| 245203 | pET-28b His6-TEV-SHP2-FL(D106P) | Production of His-tagged full-length SHP2 |
| 245204 | pET-28b His6-TEV-SHP2-FL(H114F) | Production of His-tagged full-length SHP2 |
| 245205 | pET-28b His6-TEV-SHP2-FL(H114W) | Production of His-tagged full-length SHP2 |
| 245210 | pET-28b His6-TEV-SHP2-FL(I282V) | Production of His-tagged full-length SHP2 |
| 245211 | pET-28b His6-TEV-SHP2-FL(T356L) | Production of His-tagged full-length SHP2 |
| 245212 | pET-28b His6-TEV-SHP2-FL(P424L) | Production of His-tagged full-length SHP2 |
| 245213 | pET-28b His6-TEV-SHP2-FL(T507K) | Production of His-tagged full-length SHP2 |
| 245222 | pEF myc-SHP2(F29K) | Mammalian expression of myc-tagged full-length SHP2 |
| 245224 | pEF myc-SHP2(L43F) | Mammalian expression of myc-tagged full-length SHP2 |
| 245252 | pWJ 1781 URA | Protein expression in yeast |
| 245255 | pWJ 1781 URA v-Src FL | Expression of full-length viral Src in yeast |
| 245256 | pWJ 1781 URA c-Src KD | Expression of the kinase domain of c-Src in yeast |