Deep mutational scanning of the multi-domain phosphatase SHP2 reveals mechanisms of regulation and pathogenicity.
Jiang Z, van Vlimmeren AE, Karandur D, Semmelman A, Shah NH
Nat Commun. 2025 Jul 1;16(1):5464. doi: 10.1038/s41467-025-60641-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 245199 | pET-28b His6-TEV-SHP2-FL(E69K) | Production of His-tagged full-length SHP2 |
| 245224 | pEF myc-SHP2(L43F) | Mammalian expression of myc-tagged full-length SHP2 |
| 245252 | pWJ 1781 URA | Protein expression in yeast |
| 245255 | pWJ 1781 URA v-Src FL | Expression of full-length viral Src in yeast |
| 245256 | pWJ 1781 URA c-Src KD | Expression of the kinase domain of c-Src in yeast |