Michaelis-Menten kinetics of RasGAP proteins by a rapid fluorescence-based assay.
Paul ME, Vish KJ, Boggon TJ
Methods. 2025 Dec;244:108-117. doi: 10.1016/j.ymeth.2025.09.003. Epub 2025 Sep 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 245306 | pET-HRas-1-167-WT | Expresses G-domain of human HRas (residues 1-167 wild type) in E. coli |
| 245307 | pET-p120RasGAP-714-1047-WT | Expresses GAP domain of human p120RasGAP (residues 714-1047 wild type) in E. coli |
| 246487 | pET-p120RasGAP-581-1047-WT | Expresses C2 and GAP domains of human p120RasGAP (residues 581-1047 wild type) in E. coli |