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John Doench Lab: Doench and Root CRISPR plasmids 2025

Unpublished

Plasmids from Article

ID Plasmid Purpose
245319pMV_AA097Cas12a CRISPRko positive control; targets CD46, CD47, CD55
245320pMV_AA098Cas9 CRISPRko all-in-one positive control; targets CD47
245321pMV_AA099Cas9 CRISPRko all-in-one positive control; targets CD59
245322pMV_AA100Cas9 CRISPRko all-in-one positive control; targets CD55
245323pMV_AA101Cas9 CRISPRa positive control guide; targets CD274
245324pMV_AA102Cas9 CRISPRa positive control guide; targets CD47
245326pMV_AA104Cas9 CRISPRa positive control guide; targets CD4
245327pRDA_835Cas9 CRISPRko positive control guide; targets CD59
245328pRDA_837Cas9 CRISPRko positive control guide; targets CD55
245329pRDA_887Cas12a CRISPRa cloning vector; contains NbALFA, p65
245330pRDA_908Cas12a CRISPRa positive control guide; targets CD274, ADGRE5, CD4, DPP4
245331pRDB_031Cas12a CRISPRko all-in-one positive control; targets CD47, CD63
245332pRDB_245Cas12a CRISPRko positive control guide; targets CD46
245333pRDB_246Cas12a CRISPRko positive control guide; targets CD47
245334pRDB_247Cas12a CRISPRko positive control guide; targets CD55
245335pRDB_248Cas12a CRISPRko positive control guide; targets CD63
245336pRDB_509Cas9 ABE positive control guide; targets CD274
245337pRDB_510Cas9 CBE positive control guide; targets CD274
245338pRDB_512Cas9 ABE & CBE positive control guide; targets CD47

Antibodies from Article