John Doench Lab: Doench and Root CRISPR plasmids 2025
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 245319 | pMV_AA097 | Cas12a CRISPRko positive control; targets CD46, CD47, CD55 |
| 245320 | pMV_AA098 | Cas9 CRISPRko all-in-one positive control; targets CD47 |
| 245321 | pMV_AA099 | Cas9 CRISPRko all-in-one positive control; targets CD59 |
| 245322 | pMV_AA100 | Cas9 CRISPRko all-in-one positive control; targets CD55 |
| 245323 | pMV_AA101 | Cas9 CRISPRa positive control guide; targets CD274 |
| 245324 | pMV_AA102 | Cas9 CRISPRa positive control guide; targets CD47 |
| 245326 | pMV_AA104 | Cas9 CRISPRa positive control guide; targets CD4 |
| 245327 | pRDA_835 | Cas9 CRISPRko positive control guide; targets CD59 |
| 245328 | pRDA_837 | Cas9 CRISPRko positive control guide; targets CD55 |
| 245329 | pRDA_887 | Cas12a CRISPRa cloning vector; contains NbALFA, p65 |
| 245330 | pRDA_908 | Cas12a CRISPRa positive control guide; targets CD274, ADGRE5, CD4, DPP4 |
| 245331 | pRDB_031 | Cas12a CRISPRko all-in-one positive control; targets CD47, CD63 |
| 245332 | pRDB_245 | Cas12a CRISPRko positive control guide; targets CD46 |
| 245333 | pRDB_246 | Cas12a CRISPRko positive control guide; targets CD47 |
| 245334 | pRDB_247 | Cas12a CRISPRko positive control guide; targets CD55 |
| 245335 | pRDB_248 | Cas12a CRISPRko positive control guide; targets CD63 |
| 245336 | pRDB_509 | Cas9 ABE positive control guide; targets CD274 |
| 245337 | pRDB_510 | Cas9 CBE positive control guide; targets CD274 |
| 245338 | pRDB_512 | Cas9 ABE & CBE positive control guide; targets CD47 |