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Three-Segment Protein Labeling Using a Highly Efficient and Cysteine-Less Split Intein Identified with Computational Prediction of Aggregation Properties.

Humberg C, Kroger J, Pietrokovski S, Mootz HD
Angew Chem Int Ed Engl. 2025 Oct 23:e202515821. doi: 10.1002/anie.202515821. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
245527pMAL-c2X_MBP-LcgcN(C24S)-H6Maltose binding protein N-terminally fused to His-tagged N-terminal LCGC14 PolB2split intein fragment
245528pBAD_LcgcC-GFP-H6His-tagged superfolder GFP C-terminally fused to C-terminal LCGC14 split intein fragment

Antibodies from Article