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GPR45 modulates Galpha(s) at primary cilia of the paraventricular hypothalamus to control food intake.

Xun Y, Jiang Y, Xu B, Tang M, Ludwig S, Nakamura K, Mukhopadhyay S, Liu C, Beutler B, Zhang Z
Science. 2025 Jun 5;388(6751):eadp3989. doi: 10.1126/science.adp3989. Epub 2025 Jun 5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
245976pBOB-Gpr45-3xFlagExpress 3xFlag C-terminal tagged wild-type mouse GPR45 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
245977pBOB-Gpr45-Y287C-3xFlagExpress 3xFlag C-terminal tagged mouse GPR45-Y287C mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
245978pBOB-Gpr45-S214P-3xFlagExpress 3xFlag C-terminal tagged mouse GPR45-S214P mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
245979pBOB-Gpr45-Del1-3xFlagExpress 3xFlag C-terminal tagged mouse GPR45-Del1 mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
245980pBOB-Gpr45-Del2-3xFlagExpress 3xFlag C-terminal tagged mouse GPR45-Del2 mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
245981pBOB-Gpr45-Del1&2-3xFlagExpress 3xFlag C-terminal tagged mouse GPR45-Del1&2 mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
245982pCMV-Gpr45Express wild-type mouse GPR45 transiently via transfection in mammalian cells.
245983pCMV-Gpr45-Y287CExpress mouse GPR45-Y287C mutant transiently via transfection in mammalian cells.
245984pCMV-Gpr45-S214PExpress mouse GPR45-S214P mutant transiently via transfection in mammalian cells.
245985pCMV-Gpr45-3xFlagExpress 3xFlag C-terminal tagged wild-type mouse GPR45 transiently via transfection in mammalian cells.
245986pCMV-Gpr45-Y287C-3xFlagExpress 3xFlag C-terminal tagged mouse GPR45-Y287C mutant transiently via transfection in mammalian cells.
245989pBOB-Adcy3-3xFlagExpress 3xFlag C-terminal tagged wild-type mouse ADCY3 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
245991pBOB-Mrap2-P2A-Mc4r-HAStimultaneously express HA C-terminal tagged mouse MC4R and untagged MRAP2 protein using a P2A linker, transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
245992pBOB-Gpr161-3xFlagExpress 3xFlag C-terminal tagged mouse GPR161 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
245993pBOB-Gpr45-161-3xFlagExpress 3xFlag C-terminal tagged mouse GPR45-N and GPR161-C fusion protein transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
245994pBOB-HA-GnasExpress HA N-terminal tagged mouse Galphas transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
245995pBOB-HA-GnalExpress HA N-terminal tagged mouse Galphaolf transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
245996pCMV-HA-Gnai1Express HA N-terminal tagged mouse Galphai1 transiently via transfection in mammalian cells.
245997pCMV-HA-Gnai3Express HA N-terminal tagged mouse Galphai3 transiently via transfection in mammalian cells.
245998pBOB-HA-Gnao1-AExpress HA N-terminal tagged mouse GalphaoA transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
245999pCMV-HA-Gnao1-BExpress HA N-terminal tagged mouse GalphaoB transiently via transfection in mammalian cells.
246000pBOB-HA-GnazExpress HA N-terminal tagged mouse Galphaz transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
246001pBOB-HA-GnaqExpress HA N-terminal tagged mouse Galphaq transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
246002pBOB-HA-Gna14Express HA N-terminal tagged mouse Galpha14 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
246003pBOB-HA-Gna15Express HA N-terminal tagged mouse Galpha15 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Antibodies from Article