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Reactivation of phosphorylated actin depolymerizing factor and identification of the regulatory site.

Agnew BJ, Minamide LS, Bamburg JR
J Biol Chem. 1995 Jul 21;270(29):17582-7. doi: 10.1074/jbc.270.29.17582. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
245939huCofilin S3A SSRExpresses human cofilin with mutation S3A that prevents cofilin's inactivation by phosphorylation of S3
245940huCofilin S3E SSRExpresses human cofilin with mutation S3E that behaves like inactive phosphocofilin but can be dominant negative against phosphatase
245943huADF S3AExpressing a constitutively active human ADF

Antibodies from Article