Side-by-side systematic characterization of novel FPs in budding yeast
Botman D, de Groot DH, Wehrens M, van Rossum K, Yska S, van der Kieft B, Albert V, Minghetti N, Goedhart J, Teusink B
bioRxiv 2025.06.27.661975; doi: https://doi.org/10.1101/2025.06.27.661975
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 168060 | mGreenLantern-T2A-mCherry pDRF1-GW | Produces equimolar expression of mGreenLantern and mCherry for use in yeast |
| 168061 | Cyterm-ystagRFP pDRF1-GW | Expresses Cyterm-ystagRFP to check for monomericity. |
| 168062 | Cyterm-AausFP1 pDRF1-GW | Expresses Cyterm-AausFP1 to check for monomericity. |
| 168071 | AausFP1-T2A-mCherry pdrf1-GW | Produces equimolar expression of AausFP1 and mCherry for use in yeast |
| 168072 | ystagRFP-T2A-mTq2 pdrf1-GW | Produces equimolar expression of ystagRFP and mCherry for use in yeast |
| 168075 | ymTq2-mAvicFP1 pDRF1-GW | FRET fusion between ymTq2 and mAvicFP1 for use in yeast |
| 168092 | Cyterm-mAvicFP1 pDRF1-GW | Expresses Cyterm-mAvicFP1 to check for monomericity. |
| 205491 | pDRF1-GW miRFP670-T2A-mtq2 | Expression of miRFP670-T2A-mtq2 in yeast |
| 205492 | pDRF1-GW Fold6-T2A-mCherry | Expression of Fold6-T2A-mCherry in yeast |
| 205493 | pDRF1-GW yomTagBFP2-T2A-mCherry | Expression of yomTagBFP2-T2A-mCherry in yeast |
| 205494 | pDRF1-GW mfRFP-T2A-mtq2 | Expression of mfRFP-T2A-mtq2 in yeast |
| 205495 | pDRF1-GW (n1)StayGold-T2A-mCherry | Expression of (n1)StayGold-T2A-mCherry in yeast |
| 205496 | pDRF1-GW hfYFP-T2A-mtq2 | Expression of hfYFP-T2A-mtq2 in yeast |
| 205497 | pDRF1-GW emiRFP2-T2A-mtq2 | Expression of emiRFP2-T2A-mtq2 in yeast |
| 205498 | pDRF1-GW Electra1-T2A-mCherry | Expression of Electra1-T2A-mCherry in yeast |
| 205499 | pDRF1-GW Achilles-T2A-mCherry | Expression of Achilles-T2A-mCherry in yeast |
| 205500 | pDRF1-GW ymTq2-P2A-ymScarletI | Expression of ymTq2-P2A-ymScarletI in yeast |
| 205501 | pDRF1-GW miRFp670nano3-T2A-mtq2 | Expression of miRFp670nano3-T2A-mtq2 in yeast |
| 205502 | pDRF1-GW miRFP680-T2A-mtq2 | Expression of miRFP680-T2A-mtq2 in yeast |
| 205503 | pDRF1-GW miRFP2-T2A-mtq2 | Expression of miRFP2-T2A-mtq2 in yeast |
| 205504 | pDRF1-GW miFP663-T2A-mtq2 | Expression of miFP663-T2A-mtq2 in yeast |
| 205505 | pDRF1-GW yoTagRFP657-T2A-mtq2 | Expression of yoTagRFP657-T2A-mtq2 in yeast |
| 205506 | pDRF1-GW mKelly-T2A-mtq2 | Expression of mKelly-T2A-mtq2 in yeast |
| 205508 | pDRF1-GW ymCardinal-T2A-mtq2 | Expression of ymCardinal-T2A-mtq2 in yeast |
| 205509 | pDRF1-GW ymTq2-O2A-ymScarletl | Expression of ymTq2-O2A-ymScarletl in yeast |
| 205510 | pDRF1-GW ymTq2-E2A-ymScarletl | Expression of ymTq2-E2A-ymScarletl in yeast |
| 205512 | pDRF1-GW ymTq2-ymScarletI | Expression of ymTq2-ymScarletl in yeast |
| 219829 | pDRF1 ymScarletI3_T2A_mTurquoise2 | equimolar expression of ymScarletI3 and mTurquoise2 in budding yeast |
| 219833 | pDRF1 StayGold-E138D-T2A-mCherry | equimolar expression of Staygold-E138D and mCherry in budding yeast |
| 219834 | pDRF1 mStayGold-T2A-mCherry | equimolar expression of mStaygold and mCherry in budding yeast |
| 219842 | pDRF1 mBaojin-T2A-mCherry | equimolar expression of mBaojin and mCherry in budding yeast |
| 219843 | pDRF1 ymStayGold-T2A-mCherry | equimolar expression of ymStayGold and mCherry in budding yeast |
| 219845 | pDRF1 mScarletI3-T2A-mTurquoise2 | equimolar expression of mScarletI3 and mTurquoise2 in budding yeast |
| 219847 | pDRF1 mVermillion-T2A-mTurquoise2 | equimolar expression of mVermillion and mTurquoise2 in budding yeast |
| 219848 | pDRF1 mScarlet3-T2A-mTurquoise2 | equimolar expression of mScarlet3 and mTurquoise2 in budding yeast |
| 219849 | pDRF1 mScarlet1.4-T2A-mTurquoise2 | equimolar expression of mScarlet1.4 and mTurquoise2 in budding yeast |
| 219850 | pDRF1 mGarnet2-T2A-mTurquoise2 | equimolar expression of mGarnet2 and mTurquoise2 in budding yeast |
| 219851 | pDRF1 yeBFP2-T2A-mCherry | equimolar expression of yeBFP2 and mCherry in budding yeast |
| 219853 | pDRF1 Cyterm-ytd8oxStayGold | expression of Cyterm-ytd8oxStayGold in budding yeast |
| 219854 | pDRF1 Cyterm-ymiRFP680 | expression of Cyterm-ymiRFP680 in budding yeast |
| 219855 | pDRF1 Cyterm-ymStayGold | expression of Cyterm-ymStayGoldd in budding yeast |
| 219856 | pDRF1 Cyterm-yemiRFP670 | expression of Cyterm-yemiRFP670 in budding yeast |